Serum pregnancy-associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep.

Autor: Akköse M; Department of Livestock, Dalaman Agricultural Enterprise, General Directorate of Agricultural Enterprises, Muğla, Turkey., Çınar EM; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, Turkey., Yazlık MO; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey., Kaya U; Department of Biostatistics, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey., Polat Y; Department of Medical Services and Techniques, Vocational High School of Health Services, Kilis 7 Aralık University, Kilis, Turkey., Çebi Ç; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkey., Özbeyaz C; Department of Animal Sciences, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey., Vural MR; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Jazyk: angličtina
Zdroj: Veterinary medicine and science [Vet Med Sci] 2024 Jan; Vol. 10 (1), pp. e1345.
DOI: 10.1002/vms3.1345
Abstrakt: Background: Reported as being expressed by mono- and binucleate placental cells, pregnancy-associated glycoproteins (PAGs) are released into the blood circulation from the ruminant placenta. Circulating gestational PAGs levels may differ between sheep breeds.
Objective: This study was aimed at the close monitoring of the serum PAGs profiles of Karya and Konya Merino sheep during early pregnancy.
Methods: Fifteen Karya and 15 Konya Merino ewes were synchronized by a 12-day treatment with progesterone-impregnated intravaginal sponges. After the sponges were withdrawn, the ewes were administered 400 IU of equine chorionic gonadotropin. The ewes were allowed to mate naturally, and all animals were sampled for blood as of the day of mating (day 0) at weeks 1, 2, 3, 4 and 5. Pregnancy diagnoses were made by transabdominal ultrasonography at week 5. At weeks 6, 7, 8, 9 and 11, blood samples were collected only from the pregnant ewes. The blood samples were centrifuged at 3000 × g, and extracted sera were stored at -20°C until being used for laboratory analyses. Serum PAGs levels were determined with the aid of a commercial PAG-enzyme-linked immunosorbent assay test originally developed for pregnancy diagnosis in cattle. Differences in the between the PAGs levels throughout pregnancy and the group effect (Karya and Konya Merino) were determined with a two-way mixed analysis of variance. Pairwise comparisons were made using a Bonferroni adjustment.
Results: PAGs levels showed a linear increase with the advance of pregnancy in both Karya and Konya Merino sheep. No difference was detected between the breeds for serum PAGs levels. The serum PAGs levels of the pregnant and non-pregnant ewes differed as of the fourth week.
Conclusion: The serum PAGs levels of the Karya and Konya Merino ewes were similar during the first 11 weeks of gestation, and pregnancy diagnosis could be made based on serum PAGs levels as of the 4th week in both breeds.
(© 2024 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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