Programmable fluidic networks on centrifugal microfluidic discs.

Autor: Julius LA; Fraunhofer Project Center at Dublin City University (FPC@DCU), Dublin City University, Glasnevin, Dublin 9, Ireland; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland., Torres Delgado SM; Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Lepolshafen, 76344, Germany., Mishra R; Fraunhofer Project Center at Dublin City University (FPC@DCU), Dublin City University, Glasnevin, Dublin 9, Ireland., Kent N; School of Mechanical & Manufacturing Engineering, Dublin City University, Glasnevin, Dublin 9, Ireland; National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland; Biodesign Europe, Dublin City University, Glasnevin, Dublin 9, Ireland., Carthy E; School of Mechanical & Manufacturing Engineering, Dublin City University, Glasnevin, Dublin 9, Ireland; National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland; Biodesign Europe, Dublin City University, Glasnevin, Dublin 9, Ireland., Korvink JG; Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Lepolshafen, 76344, Germany., Mager D; Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Lepolshafen, 76344, Germany., Ducrée J; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland; National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland; Biodesign Europe, Dublin City University, Glasnevin, Dublin 9, Ireland., Kinahan DJ; School of Mechanical & Manufacturing Engineering, Dublin City University, Glasnevin, Dublin 9, Ireland; National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland; Biodesign Europe, Dublin City University, Glasnevin, Dublin 9, Ireland; I-Form, The SFI Research Centre for Advanced Manufacturing, Dublin City University, Dublin 9, Ireland. Electronic address: david.kinahan@dcu.ie.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Feb 01; Vol. 1288, pp. 342159. Date of Electronic Publication: 2023 Dec 18.
DOI: 10.1016/j.aca.2023.342159
Abstrakt: Background: Biomedical diagnostic and lab automation solutions built on the Lab-on-a-Disc (LoaD) platform has great potential due to their independence from specialised micro-pumps and their ease of integration, through direct pipetting, with manual or automated workflows. However, a challenge for all microfluidic chips is their cost of manufacture when each microfluidic disc must be customized for a specific application. In this paper, we present centrifugal discs with programmable fluidic networks.
Results: Based on dissolvable film valves, we present two technologies. The first, based on recently introduced pulse-actuated dissolvable film valves, is a centrifugal disc which, depending on how it is loaded, is configured to perform either six sequential reagent releases through one reaction chamber or three sequential reagent releases through two reaction chambers. In the second approach, we use the previously introduced electronic Lab-on-a-Disc (eLoaD) wireless valve array, which can actuate up to 128 centrifugo-pneumatic dissolvable film valves in a pre-defined sequence. In this approach we present a disc which can deliver any one of 8 reagent washes to any one of four reaction chambers. We use identical discs to demonstrate the first four sequential washes through two reaction chambers and then two sequential washes through four reaction chambers.
Significance: These programmable fluidic networks have the potential to allow a single disc architecture to be applied to multiple different assay types and so can offer a lower-cost and more integrated alternative to the standard combination of micro-titre plate and liquid handling robot. Indeed, it may even be possible to conduct multiple different assays concurrently. This can have the effect of reducing manufacturing costs and streamlining supply-chains and so results in a more accessible diagnostic platform.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jan Korvink reports financial support was provided by European Commission. Jens Ducree reports financial support was provided by European Commission. Jens Ducree reports financial support was provided by Science Foundation Ireland. Sarai Torres Delgado reports financial support was provided by National Council of Science and Technology. Jens Ducree reports financial support was provided by Fraunhofer Society for the Advancement of Applied Research.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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