Antibacterial activity of mulberry extracts and purified fractions against oral pathogenic bacteria.

Autor: Shirai T; Faculty of Dentistry, Ohu University, 31-1 Misumido, Tomita-cho, Koriyama-city, Fukusima, Japan., Satoh Y; Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University, 1-1-1 Tsushima-naka, Kita-ku, Okayama-city, Okayama, Japan; Department of Microbiology, Tokyo Dental College, 2-9-18 Kandamisaki-cho, Chiyoda-ku, Tokyo, Japan. Electronic address: yutarohsatoh@infant-music.com., Ishihara K; Department of Microbiology, Tokyo Dental College, 2-9-18 Kandamisaki-cho, Chiyoda-ku, Tokyo, Japan.
Jazyk: angličtina
Zdroj: Journal of oral biosciences [J Oral Biosci] 2024 Jun; Vol. 66 (2), pp. 439-446. Date of Electronic Publication: 2024 Jan 14.
DOI: 10.1016/j.job.2023.12.009
Abstrakt: Objectives: This study aimed to isolate antibacterial compounds active against periodontopathic bacteria from mulberry (Morus alba) leaves.
Methods: The acetone-soluble fraction of mulberry leaves was extracted from the oil layer by oil/water separation. The extract was purified using silica gel open-column chromatography. The minimum inhibitory concentration (MIC) of the crude extract or purified fractions against Porphyromonas gingivalis was measured at each step.
Results: The MIC of the crude extract against P. gingivalis was 62.5-125 μg/mL. The fractions showing activity against P. gingivalis were designated Cf K and Cf P. The MICs of Cf K against P. gingivalis, Fusobacterium nucleatum, Prevotella intermedia, and Streptococcus mutans were 6.25 μg/mL, 25 μg/mL, 12.5 μg/mL, and 12.5 μg/mL, respectively. In contrast, the MICs of Cf P against P. gingivalis, F. nucleatum, P. intermedia, and S. mutans were 25.0 μg/mL, >50 μg/mL, 50 μg/mL, and 12.5-25.0 μg/mL, respectively.
Conclusions: Mulberry leaves contain antibacterial components against periodontopathic bacteria such as P. gingivalis, F. nucleatum, and P. intermedia.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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