Metal-organic framework-based multienzyme cascade bioreactor for sensitive detection of methyl parathion.

Autor: Chen D; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Wang L; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Wei J; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Jiao T; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Chen Q; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Oyama M; Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8520, Japan., Chen Q; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Chen X; State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China., Chen X; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen 361021, China. Electronic address: xmchen@jmu.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Jun 01; Vol. 442, pp. 138389. Date of Electronic Publication: 2024 Jan 11.
DOI: 10.1016/j.foodchem.2024.138389
Abstrakt: In this study, a cascade nanobioreactor was developed for the highly sensitive detection of methyl parathion (MP) in food samples. The simultaneous encapsulation of acetylcholinesterase (AChE) and choline oxidase (CHO) in a zeolitic imidazole ester backbone (ZIF-8) effectively improved the stability and cascade catalytic efficiency of the enzymes. In addition, glutathione-stabilized gold nanoclusters (GSH-AuNCs) were encapsulated in ZIF-8 by ligand self-assembly, conferring excellent fluorescence properties. Acetylcholine (ATCh) is catalyzed by a cascade of AChE/CHO@ZIF-8 as well as Fe(II) to generate hydroxyl radicals (·OH) with strong oxidizing properties. The ·OH radicals then oxidize Au(0) in GSH-AuNCs@ZIF-8 to Au(I), resulting in fluorescence quenching. MP, as an inhibitor of AChE, hinders the cascade reaction and thus restores the fluorescence emission, enabling its quantitative detection. The limit of detection of the constructed nanobioreactor for MP was 0.23 µg/L. This MOF-based cascade nanobioreactor has great potential for the detection of trace hazards.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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