Superior haplotypes of key drought-responsive genes reveal opportunities for the development of climate-resilient rice varieties.

Autor: Singh P; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India., Sundaram KT; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India., Vinukonda VP; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India., Venkateshwarlu C; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India., Paul PJ; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India., Pahi B; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India., Gurjar A; International Rice Research Institute, South Asia Regional Centre (ISARC), Varanasi, India., Singh UM; International Rice Research Institute, South Asia Regional Centre (ISARC), Varanasi, India., Kalia S; Department of Biotechnology, CGO Complex, Lodhi Road, New Delhi, India., Kumar A; International Rice Research Institute, South Asia Regional Centre (ISARC), Varanasi, India.; International Crops Research Institute for the Semi-Arid Tropics, Hyderabad, India., Singh VK; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India. v.k.singh@irri.org., Sinha P; International Rice Research Institute (IRRI), South-Asia Hub, Hyderabad, India. p.sinha@irri.org.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Jan 12; Vol. 7 (1), pp. 89. Date of Electronic Publication: 2024 Jan 12.
DOI: 10.1038/s42003-024-05769-7
Abstrakt: Haplotype-based breeding is an emerging and innovative concept that enables the development of designer crop varieties by exploiting and exploring superior alleles/haplotypes among target genes to create new traits in breeding programs. In this regard, whole-genome re-sequencing of 399 genotypes (landraces and breeding lines) from the 3000 rice genomes panel (3K-RG) is mined to identify the superior haplotypes for 95 drought-responsive candidate genes. Candidate gene-based association analysis reveals 69 marker-trait associations (MTAs) in 16 genes for single plant yield (SPY) under drought stress. Haplo-pheno analysis of these 16 genes identifies superior haplotypes for seven genes associated with the higher SPY under drought stress. Our study reveals that the performance of lines possessing superior haplotypes is significantly higher (p ≤ 0.05) as measured by single plant yield (SPY), for the OsGSK1-H4, OsDSR2-H3, OsDIL1-H22, OsDREB1C-H3, ASR3-H88, DSM3-H4 and ZFP182-H4 genes as compared to lines without the superior haplotypes. The validation results indicate that a superior haplotype for the DREB transcription factor (OsDREB1C) is present in all the drought-tolerant rice varieties, while it was notably absent in all susceptible varieties. These lines carrying the superior haplotypes can be used as potential donors in haplotype-based breeding to develop high-yielding drought-tolerant rice varieties.
(© 2024. The Author(s).)
Databáze: MEDLINE
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