| Autor: |
Bodie AR; Meat Science and Animal Biologics Discovery Program, Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA., Dittoe DK; Department of Animal Science, University of Wyoming, Laramie, WY 82071, USA., Applegate SF; Hygiena, 2 Boulden Circle, New Castle, DE 19720, USA., Stephens TP; Hygiena, 2 Boulden Circle, New Castle, DE 19720, USA., Ricke SC; Meat Science and Animal Biologics Discovery Program, Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Foods (Basel, Switzerland) [Foods] 2023 Dec 22; Vol. 13 (1). Date of Electronic Publication: 2023 Dec 22. |
| DOI: |
10.3390/foods13010056 |
| Abstrakt: |
Poultry is the primary reservoir of Campylobacter , a leading cause of gastroenteritis in the United States. Currently, the selective plating methodology using selective agars, Campy Cefex and Modified Charcoal Cefoperazone Deoxycholate agar, is preferentially used for the quantification of Campylobacter spp. among poultry products. Due to the specific nature of Campylobacter , this methodology is not sensitive, which can lead to skewed detection and quantification results. Therefore, Campylobacter detection and quantification methods are urgently needed. The objective was to develop a shortened enrichment-based quantification method for Campylobacter (CampyQuant™) in post-chill poultry rinsates using the BAX ® System Real-Time PCR assay for Campylobacter . The specificity and sensitivity for the detection of C. jejuni , C. coli , and C. lari in pure culture were determined. The BAX ® System Real-Time PCR assay consistently detected and identified each species 100% of the time with an enumeration range of 4.00 to 9.00 Log 10 CFU/mL. Enrichment time parameters for low-level concentrations (0.00, 1.00, and 2.00 Log 10 CFU/mL) of Campylobacter using the BAX ® System Real-Time PCR assay were elucidated. It was determined that an enrichment time of 20 h was needed to detect at least 1.00 Log 10 CFU/mL of Campylobacter spp. Using the BAX ® System Real-Time PCR assay for Campylobacter . As a result, time of detection, detection limits, and enrichment parameters were used to develop the CampyQuant™ linear standard curve using the detected samples from the BAX ® System Real-Time PCR assay to quantify the levels in post-chill poultry rinsates. A linear fit equation was generated for each Campylobacter species using the cycle threshold from the BAX ® System Real-Time PCR assay to estimate a pre-enrichment of 1.00 to 4.00 Log 10 CFU/mL of rinsates detected. The statistical analyses of each equation yielded an R 2 of 0.93, 0.76, and 0.94 with a Log 10 RMSE of 0.64, 1.09, and 0.81 from C. jejuni , C. coli , and C. lari , respectively. The study suggests that the BAX ® System Real-Time PCR assay for Campylobacter is a more rapid, accurate, and efficient alternative method for Campylobacter enumeration. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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