Antifungal Activity Enhancement of Cell-Free Streptomyces griseus Extract Obtained by Fermentation with Magnetic Manganese Ferrite Nanoparticles.

Autor: Farias-Vazquez LS; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México., Ramos-González R; CONAHCYT - Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México. rodolfo.ramos@uadec.edu.mx., Pacios-Michelena S; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México., Aguilar CN; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México., Arredondo-Valdés R; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México., Rodríguez-Herrera R; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México., Martínez-Hernández JL; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México., Segura-Ceniceros EP; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México., Ilyina A; Facultad de Ciencias Químicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, México. annailina@uadec.edu.mx.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2024 Jan 06. Date of Electronic Publication: 2024 Jan 06.
DOI: 10.1007/s12010-023-04851-w
Abstrakt: The present study aims to obtain manganese ferrite nanoparticles functionalized with chitosan (C-MNP) or ethylenediamine (E-MNP) by coprecipitation and polyol one-step methods, characterize their interaction with S. griseus demonstrating cell immobilization, and evaluate the antimicrobial activity of the free cell extracts obtained from immobilized S. griseus fermentation in the presence of different concentrations of MNP. The adsorption isotherms were analyzed mathematically using Langmuir and Freundlich models. The highest coefficient of determination (R 2 ) for the S. griseus cell adsorption isotherm with C-MNP was observed with a linear function of the Langmuir model. The adsorption isotherm of S. griseus cells with E-MNP was better fitted to the Freundlich model. Cell immobilization by adsorption on magnetic nanoparticles was demonstrated in both cases. Different concentrations of C-MNP and E-MNP were used in fermentations to prepare cell-free extracts with antifungal activity. The best results were obtained with E-MNP, with a 91.5% inhibition of radial fungal growth. Magnetic nanoparticles offer potential applications in different fields and easy biomass separation.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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