Biosynthesis of Zinc Oxide Nanoparticles Using Bacillus paramycoides for In Vitro Biological Activities and In Vivo Assessment Against Hepatorenal Injury Induced by CCl 4 in Rats.

Autor: El-Refai HA; Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt., Saleh AM; Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt., Mohamed SIA; Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt., Aboul Naser AF; Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt., Zaki RA; Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt., Gomaa SK; Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt., Hamed MA; Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt. manal_hamed@yahoo.com.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2024 Jan 04. Date of Electronic Publication: 2024 Jan 04.
DOI: 10.1007/s12010-023-04817-y
Abstrakt: Recently, impressive developments in the field of nanotechnology have been achieved. The study aimed to synthetize zinc oxide nanoparticles (ZnONPs) from locally isolated terrestrial Bacillus paramycoides (MCCC 1A04098) bacteria and assess its role as antioxidant, antimicrobial, and anticancer agent. The antioxidant activity was done using the percentage of DPPH scavenging method. The antibacterial activity was evaluated against Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Candida albicans. The anti-proliferation assay against hepatocellular carcinoma (HepG2) and human breast cancer (MCF-7) cell lines was estimated by neutral red assay. The apoptotic effect of ZnONP was measured by flow cytometry. The in vivo evaluation was carried out against hepatorenal injuries induced by carbon tetrachloride (CCl 4 ) in rats comparing with silymarin as a reference drug. The oxidative stress markers, liver and kidney function enzyme indices, lipid profile, and the histological features of the liver and kidney were also examined. ZnONPs revealed antioxidant and antibacterial effects. It also exerted cytotoxic and apoptotic effect in a dose dependent manner without any toxicity on normal cell line. ZnONPs improved all the biochemical parameters under investigation to varying degrees, and the histological pictures of the liver and kidney confirmed the results. In conclusion, ZnONPs were successfully synthesized from the terrestrial Bacillus paramycoides and recorded in vitro antioxidant, anticancer, and antibacterial effects as well as in vivo anti-hepatorenal toxicity effects.
(© 2024. The Author(s).)
Databáze: MEDLINE
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