Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species.

Autor: Triesch S; Institute for Plant Biochemistry, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.; Cluster of Excellence on Plant Sciences (CEPLAS), Düsseldorf, Germany., Denton AK; Institute for Plant Biochemistry, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.; Cluster of Excellence on Plant Sciences (CEPLAS), Düsseldorf, Germany., Bouvier JW; Department of Biology, University of Oxford, Oxford, UK., Buchmann JP; Cluster of Excellence on Plant Sciences (CEPLAS), Düsseldorf, Germany.; Institute for Biological Data Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Reichel-Deland V; Institute for Plant Biochemistry, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Guerreiro RNFM; Institute for Quantitative Genetics and Genomics of Plants, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Busch N; Institute for Plant Biochemistry, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Schlüter U; Institute for Plant Biochemistry, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.; Cluster of Excellence on Plant Sciences (CEPLAS), Düsseldorf, Germany., Stich B; Cluster of Excellence on Plant Sciences (CEPLAS), Düsseldorf, Germany.; Institute for Quantitative Genetics and Genomics of Plants, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Kelly S; Department of Biology, University of Oxford, Oxford, UK., Weber APM; Institute for Plant Biochemistry, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.; Cluster of Excellence on Plant Sciences (CEPLAS), Düsseldorf, Germany.
Jazyk: angličtina
Zdroj: Plant biology (Stuttgart, Germany) [Plant Biol (Stuttg)] 2024 Mar; Vol. 26 (2), pp. 270-281. Date of Electronic Publication: 2024 Jan 03.
DOI: 10.1111/plb.13601
Abstrakt: C 3 -C 4 intermediate photosynthesis has evolved at least five times convergently in the Brassicaceae, despite this family lacking bona fide C 4 species. The establishment of this carbon concentrating mechanism is known to require a complex suite of ultrastructural modifications, as well as changes in spatial expression patterns, which are both thought to be underpinned by a reconfiguration of existing gene-regulatory networks. However, to date, the mechanisms which underpin the reconfiguration of these gene networks are largely unknown. In this study, we used a pan-genomic association approach to identify genomic features that could confer differential gene expression towards the C 3 -C 4 intermediate state by analysing eight C 3 species and seven C 3 -C 4 species from five independent origins in the Brassicaceae. We found a strong correlation between transposable element (TE) insertions in cis-regulatory regions and C 3 -C 4 intermediacy. Specifically, our study revealed 113 gene models in which the presence of a TE within a gene correlates with C 3 -C 4 intermediate photosynthesis. In this set, genes involved in the photorespiratory glycine shuttle are enriched, including the glycine decarboxylase P-protein whose expression domain undergoes a spatial shift during the transition to C 3 -C 4 photosynthesis. When further interrogating this gene, we discovered independent TE insertions in its upstream region which we conclude to be responsible for causing the spatial shift in GLDP1 gene expression. Our findings hint at a pivotal role of TEs in the evolution of C 3 -C 4 intermediacy, especially in mediating differential spatial gene expression.
(© 2024 The Authors. Plant Biology published by John Wiley & Sons Ltd on behalf of German Society for Plant Sciences, Royal Botanical Society of the Netherlands.)
Databáze: MEDLINE
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