Influence of CK2 protein kinase activity on the interaction between Trypanosoma cruzi and its vertebrate and invertebrate hosts.

Autor: de Oliveira Souza JE; Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology (FCM), State University of Rio de Janeiro, Rio de Janeiro, Brazil., Gomes SMR; Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology (FCM), State University of Rio de Janeiro, Rio de Janeiro, Brazil., Lima AKC; Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology (FCM), State University of Rio de Janeiro, Rio de Janeiro, Brazil., de Souza Brito AC; Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology (FCM), State University of Rio de Janeiro, Rio de Janeiro, Brazil., Da-Silva SAG; Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology (FCM), State University of Rio de Janeiro, Rio de Janeiro, Brazil., de Carvalho Santos Lopes AH; Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Silva-Neto MAC; Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Atella GC; Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Dutra PML; Discipline of Parasitology, Department of Microbiology, Immunology and Parasitology (FCM), State University of Rio de Janeiro, Rio de Janeiro, Brazil. pmldutra@gmail.com.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2024 Jan 02; Vol. 123 (1), pp. 80. Date of Electronic Publication: 2024 Jan 02.
DOI: 10.1007/s00436-023-08085-x
Abstrakt: Chagas disease, endemic from Latin America, is caused by Trypanosoma cruzi and is transmitted by triatomine feces. This parasite undergoes complex morphological changes through its life cycle, promoted by significant changes in signal transduction pathways. The activity of protein kinase CK2 has been described in trypanosomatids. Using a specific peptide and radioactive ATP, we identified CK2 activity on the cellular surface and the cytoplasmic content in Trypanosoma cruzi, apart from the secreted form. Dephosphorylated casein promoted an increase of 48% in the secreted CK2 activity. Total extract of peritoneal macrophages from BALB/c and inactivated human serum promoted an increase of 67% and 36%, respectively, in this activity. The protein secreted by parasites was purified by HPLC and had shown compatibility with the catalytic subunit of mammalian CK2. Incubation of the parasites with CK2 inhibitors, added to the culture medium, prevented their growth. The opposite was observed when CK2 activators were used. Results of interaction between Trypanosoma cruzi and the gut of the vector have revealed that, in the presence of CK2 inhibitors, there is a reduction in the association rate. A similar inhibition profile was seen in the Trypanosoma cruzi-macrophages interaction, confirming the importance of this enzyme in the life cycle of this protozoan.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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