Bacterial microbiome changes after fecal transplantation for recurrent Clostridioides difficile infection in the Brazilian center.

Autor: Terra DAA; Alfa Institute of Gastroenterology of Clinic Hospital of Federal University of Minas Gerais, Minas Gerais, Belo Horizonte, Brazil., de Oliveira Carvalho RD; Laboratory of Cellular and Molecular Genetics, Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.; Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Salvador Belo Horizonte, Brazil., da Silva TF; Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Salvador Belo Horizonte, Brazil., Dos Santos Freitas A; Laboratory of Cellular and Molecular Genetics, Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil., Góes-Neto A; Laboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil., Amarante VS; Anaerobic Laboratory, Veterinary Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Azevedo V; Laboratory of Cellular and Molecular Genetics, Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil., Vilela EG; Alfa Institute of Gastroenterology of Clinic Hospital of Federal University of Minas Gerais, Minas Gerais, Belo Horizonte, Brazil., Coelho LGV; Alfa Institute of Gastroenterology of Clinic Hospital of Federal University of Minas Gerais, Minas Gerais, Belo Horizonte, Brazil., Silva ROS; Anaerobic Laboratory, Veterinary Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. rodrigo.otaviosilva@gmail.com.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Mar; Vol. 55 (1), pp. 719-725. Date of Electronic Publication: 2023 Dec 29.
DOI: 10.1007/s42770-023-01227-4
Abstrakt: Clostridioides difficile infection (CDI) poses a significant global health threat owing to its substantial morbidity and associated healthcare costs. A key challenge in controlling CDI is the risk of multiple recurrences, which can affect up to 30% of patients. In such instances, fecal microbiota transplantation (FMT) is increasingly recognized as the optimal treatment. However, few related studies have been conducted in developing countries, and the microbiota composition of Brazilian patients and its dynamic modification post-FMT remain largely unexplored. This study aimed to evaluate the changes in the bacterial gut microbiome in Brazilian patients with recurrent CDI post-FMT. Ten patients underwent FMT, and the primary and overall CDI resolution rates were 80% and 90% after the first and second FMT, respectively. FMT was associated with an early increase in Shannon's diversity, evident as soon as 1 week post-FMT and persisting for at least 25 days post-treatment. Post-treatment, the abundance of Firmicutes increased and that of Proteobacteria decreased. Specifically, the abundance of the genera Ruminococcus, Faecalibacterium, Lachnospira, and Roseburia of the Firmicutes phylum was significantly higher 1 week post-transplantation, with Ruminococcus and Faecalibacterium remaining enriched 25 days post-transplantation. This study is the first of its kind in Brazil to evaluate the microbiota of a donor and patients undergoing FMT. Our findings suggest that FMT can induce remarkable changes in the gut microbiota, characterized by an early and sustained increase in diversity lasting at least 25 days. FMT also promotes enrichment of genera such as Ruminococcus spp., Faecalibacterium spp., and Roseburia spp., essential for therapeutic success.
(© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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