The use of recombinant K39, KMP11, and crude antigen-based indirect ELISA as a serological diagnostic tool and a measure of exposure for cutaneous leishmaniasis in Sri Lanka.

Autor: Karunathilake C; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Alles N; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Dewasurendra R; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Weerasinghe I; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Chandrasiri N; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Piyasiri SB; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Samaranayake N; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Silva H; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka., Manamperi N; Faculty of Medicine, Department of Parasitology, University of Kelaniya, Ragama, Sri Lanka., Karunaweera N; Faculty of Medicine, Department of Parasitology, University of Colombo, Colombo, Sri Lanka. nadira@parasit.cmb.ac.lk.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2023 Dec 29; Vol. 123 (1), pp. 77. Date of Electronic Publication: 2023 Dec 29.
DOI: 10.1007/s00436-023-08103-y
Abstrakt: Cutaneous leishmaniasis (CL) in Sri Lanka is caused by Leishmania donovani, a parasite widely known to cause visceral leishmaniasis. Despite the fact that CL is not generally believed to elicit serological immune responses, recent studies show the presence of antibody responses against this atypical form of CL. This study assesses the potential of using recombinant K39 (rK39), KMP11, and crude parasite antigen-based indirect ELISAs as serological diagnostic tools and measures of exposure for CL in Sri Lanka. The study used serum samples from confirmed CL patients (n = 266) and apparently healthy individuals from endemic settings (n = 411). Serum samples from individuals residing in non-endemic areas were used as negative controls. In-house indirect ELISAs were optimized and validated for recombinant antigens. Previously validated crude parasite extract-based indirect ELISA was performed for comparison. The statistical analyses were performed using SPSS v26.0. The rK39 (sensitivity = 71.2%, specificity = 64%) and KMP11 (sensitivity = 79.2%, specificity = 71.4%) based indirect ELISA were shown to be less suitable for the diagnosis of CL, while crude parasite extract-based indirect ELISA (sensitivity = 82.4%, specificity = 85.7%) might be a better method of diagnosis. All 03 ELISAs seemed to be good methods as measures of exposure since correlations were observed between the seropositivity of all 03 ELISAs (rK39: p = 0.037, KMP11: p = 0.007, CrudeAg: p = 0.000) with provincial case incidences. The findings will be important in identifying the disease hotspots in order to design the control measures for CL induced by L. donovani in Sri Lanka.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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