Cytotoxic Activity of Saponins and Sapogenins Isolated from Chenopodium quinoa Willd. in Cancer Cell Lines.

Autor: Carpio-Paucar GN; MCPHS University, 179 Longwood Avenue, Boston, MA 02115, USA., Palo-Cardenas AI; MCPHS University, 179 Longwood Avenue, Boston, MA 02115, USA., Rondón-Ortiz AN; MCPHS University, 179 Longwood Avenue, Boston, MA 02115, USA., Pino-Figueroa A; MCPHS University, 179 Longwood Avenue, Boston, MA 02115, USA., Gonzales-Condori EG; Universidad Tecnológica del Perú (UTP), Av. Tacna y Arica 160, Arequipa, Peru., Villanueva-Salas JA; Universidad Católica de Santa María, Urb. San Jose s/n, Arequipa, Peru.
Jazyk: angličtina
Zdroj: Scientifica [Scientifica (Cairo)] 2023 Dec 18; Vol. 2023, pp. 8846387. Date of Electronic Publication: 2023 Dec 18 (Print Publication: 2023).
DOI: 10.1155/2023/8846387
Abstrakt: The cytotoxic properties of two extracts from Chenopodium quinoa Willd. and three synthetic sapogenins were evaluated in different cancer cell lines (A549, SH-SY5Y, HepG2, and HeLa) to investigate their cytotoxic effects and determine if these cell lines activate the caspase pathway for apoptosis in response to saponin and sapogenin treatment. The saponin extracts were isolated from the agro-industrial waste of Chenopodium quinoa Willd., while the sapogenins were identified and quantitatively determined by High-Performance Liquid Chromatography (HPLC). Among these compounds, ursolic acid was the most active compound, with high IC 50 values measured in all cell lines. In addition, hederagenin demonstrated higher caspase-3 activity than staurosporine in HeLa cells, suggesting an anti-cytotoxic activity via a caspase-dependent apoptosis pathway. HPLC analysis showed that the concentration of hederagenin was higher than that of oleanolic acid in ethanolic extracts of white and red quinoa. The ethanolic extracts of white and red quinoa did not show cytotoxic activity. On the other hand, the synthetic sapogenins such as ursolic acid, oleanolic acid, and hederagenin significantly decreased the viability of the four cell lines studied. Finally, by Caspase-3 assay, it was found that HeLa undergoes apoptosis during cell death because hederagenin produces a significant increase in PARP-1 hydrolysis in HeLa cells.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2023 Genesis N. Carpio-Paucar et al.)
Databáze: MEDLINE
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