Genetic variability of Dirofilaria repens isolates from humans and dogs in Italy.

Autor: Gabrielli S; Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy., Brustenga L; Department of Veterinary Medicine, University of Perugia, Perugia, Italy., Morganti G; Department of Veterinary Medicine, University of Perugia, Perugia, Italy., Ciuca L; Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naple, Italy., Barlozzari G; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome, Italy., Rigamonti G; Department of Veterinary Medicine, University of Perugia, Perugia, Italy., Orlandi M; Freelance Veterinarian for MyLav - La Vallonea, Laboratorio di Analisi Veterinarie s.r.l. Passirana di Rho (MI), Italy., Sforna M; Department of Veterinary Medicine, University of Perugia, Perugia, Italy. Electronic address: monica.sforna@unipg.it., Veronesi F; Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2024 Feb; Vol. 326, pp. 110096. Date of Electronic Publication: 2023 Dec 12.
DOI: 10.1016/j.vetpar.2023.110096
Abstrakt: Dirofilaria repens is a paradigmatic example of an emerging vector-borne pathogen (VBP) in both human and veterinary fields. The spatial expansion and the increasing zoonotic impact of this VBP can be related to several drivers including the genetic structure of parasite populations. Italy is one of the European countries traditionally endemic with the highest incidence of canine and human cases of subcutaneous dirofilariosis. The present study aimed to assess the genetic identity and variability of D. repens isolates of human and canine origin from areas of Central Italy, compared with those isolated from different areas of Europe by sequence analysis of mtDNA genes (i.e., 12 S rDNA and cox1). A total of twenty isolates of D. repens were obtained from biopsies of subcutaneous and ocular cases of dirofilariosis occurring in 10 dogs and 10 humans. The sequence analysis of 12 S rDNA showed that all the sequences obtained clustered as a monophyletic group with a strong nodal support, indicating that all sequence types represented D. repens. The cox1 and the 12 S sequence analysis did not show host-related polymorphisms between human and dog-derived specimens. The sequence analysis of cox1 was performed including 8 additional sequences previously obtained from human and canine isolates in the same areas. Out of the 28 sequences analyzed, 20 were grouped in a haplogroup comprising 15 haplotypes (i.e., DR1, DR2, DR4, DR5, DR7, DR8, DR10-DR18), 2 sequences matched to DR9, reported for the first time in Italy, and 6 showed peculiar polymorphisms that were not previously described. The results obtained have implications for a better understanding of the epidemiology and phylogeography of this emerging vector-borne zoonotic parasite.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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