| Autor: |
Käbisch L; Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.; Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.; Institute for Veterinary Food Science, Department of Veterinary Medicine, Justus Liebig University Giessen, 35392 Giessen, Germany., Schink AK; LDG Laboratory Diagnostics Germany GmbH, 27472 Cuxhaven, Germany., Hoeltig D; Division for Pigs, Farm Animal Clinic, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany., Verspohl J; Institute of Microbiology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany., Gyuranecz M; HUN-REN Veterinary Medical Research Institute, H-1143 Budapest, Hungary.; MolliScience Kft., H-2051 Biatorbágy, Hungary., Spergser J; Institute of Microbiology, University of Veterinary Medicine, 1210 Vienna, Austria., Kehrenberg C; Institute for Veterinary Food Science, Department of Veterinary Medicine, Justus Liebig University Giessen, 35392 Giessen, Germany., Schwarz S; Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.; Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany. |
| Abstrakt: |
Organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee of Antimicrobial Susceptibility Testing (EUCAST) provide standardized methodologies for antimicrobial susceptibility testing of a wide range of nonfastidious and fastidious bacteria, but so far not for Mycoplasma spp. of animal origin. Recently, a proposed method for the standardized broth microdilution testing of Mycoplasma hyorhinis using commercial Sensititre microtiter plates was presented. In this study, we evaluated this broth microdilution method with 37 field isolates and tested their susceptibility toward the following antimicrobial agents: doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin. The isolates originated from different countries, isolation sites, and years. The broth microdilution method was carried out using a modified Friis broth as the culture and test medium. For macrolides and lincosamides, a bimodal distribution with elevated MIC values could be observed for almost half of the tested field isolates, deducing reduced susceptibility toward these substances. With a recently published protocol, we were able to test a variety of field isolates, and consistent data could be obtained. Using this method, monitoring studies of Mycoplasma hyorhinis isolates can be carried out in a comparable manner, and the observed susceptibility profiles can be screened for possible changes in MIC values in the future. |
