Extracellular Proteins Isolated from L. acidophilus as an Osteomicrobiological Therapeutic Agent to Reduce Pathogenic Biofilm Formation, Regulate Chronic Inflammation, and Augment Bone Formation In Vitro.

Autor: Pugazhendhi AS; Biionix Cluster, University of Central Florida, Orlando, FL, 32827, USA., Seal A; Biionix Cluster, University of Central Florida, Orlando, FL, 32827, USA., Hughes M; Cardiff University, Cardiff, WA, CF10 3AT, UK., Kumar U; Advanced Materials Processing and Analysis Centre, Nanoscience Technology Center (NSTC), University of Central Florida, Orlando, FL, 32826, USA., Kolanthai E; Advanced Materials Processing and Analysis Centre, Nanoscience Technology Center (NSTC), University of Central Florida, Orlando, FL, 32826, USA., Wei F; Biionix Cluster, University of Central Florida, Orlando, FL, 32827, USA., Schwartzman JD; College of Medicine, University of Central Florida, Orlando, FL, 32827, USA., Coathup MJ; Biionix Cluster, University of Central Florida, Orlando, FL, 32827, USA.; College of Medicine, University of Central Florida, Orlando, FL, 32827, USA.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Apr; Vol. 13 (9), pp. e2302835. Date of Electronic Publication: 2023 Dec 28.
DOI: 10.1002/adhm.202302835
Abstrakt: Periprosthetic joint infection (PJI) is a challenging complication that can occur following joint replacement surgery. Efficacious strategies to prevent and treat PJI and its recurrence remain elusive. Commensal bacteria within the gut convey beneficial effects through a defense strategy named "colonization resistance" thereby preventing pathogenic infection along the intestinal surface. This blueprint may be applicable to PJI. The aim is to investigate Lactobacillus acidophilus spp. and their isolated extracellular-derived proteins (LaEPs) on PJI-relevant Staphylococcus aureus, methicillin-resistant S. aureus, and Escherichia coli planktonic growth and biofilm formation in vitro. The effect of LaEPs on cultured macrophages and osteogenic, and adipogenic human bone marrow-derived mesenchymal stem cell differentiation is analyzed. Data show electrostatically-induced probiotic-pathogen species co-aggregation and pathogenic growth inhibition together with LaEP-induced biofilm prevention. LaEPs prime macrophages for enhanced microbial phagocytosis via cathepsin K, reduce lipopolysaccharide-induced DNA damage and receptor activator nuclear factor-kappa B ligand expression, and promote a reparative M2 macrophage morphology under chronic inflammatory conditions. LaEPs also significantly augment bone deposition while abating adipogenesis thus holding promise as a potential multimodal therapeutic strategy. Proteomic analyses highlight high abundance of lysyl endopeptidase, and urocanate reductase. Further, in vivo analyses are warranted to elucidate their role in the prevention and treatment of PJIs.
(© 2023 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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