Biophysical basis of filamentous phage tactoid-mediated antibiotic tolerance in P. aeruginosa.

Autor: Böhning J; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK., Graham M; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK.; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Letham SC; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK.; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Davis LK; Department of Mathematics, University College London, London, WC1H 0AY, UK.; Institute for the Physics of Living Systems, University College London, London, WC1E 6BT, UK., Schulze U; Cell Biology Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK., Stansfeld PJ; School of Life Sciences & Department of Chemistry, University of Warwick, Coventry, UK., Corey RA; Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.; School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, BS8 1TD, UK., Pearce P; Department of Mathematics, University College London, London, WC1H 0AY, UK.; Institute for the Physics of Living Systems, University College London, London, WC1E 6BT, UK., Tarafder AK; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK. atarafder@mrc-lmb.cam.ac.uk., Bharat TAM; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK. tbharat@mrc-lmb.cam.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Dec 19; Vol. 14 (1), pp. 8429. Date of Electronic Publication: 2023 Dec 19.
DOI: 10.1038/s41467-023-44160-8
Abstrakt: Inoviruses are filamentous phages infecting numerous prokaryotic phyla. Inoviruses can self-assemble into mesoscale structures with liquid-crystalline order, termed tactoids, which protect bacterial cells in Pseudomonas aeruginosa biofilms from antibiotics. Here, we investigate the structural, biophysical, and protective properties of tactoids formed by the P. aeruginosa phage Pf4 and Escherichia coli phage fd. A cryo-EM structure of the capsid from fd revealed distinct biochemical properties compared to Pf4. Fd and Pf4 formed tactoids with different morphologies that arise from differing phage geometries and packing densities, which in turn gave rise to different tactoid emergent properties. Finally, we showed that tactoids formed by either phage protect rod-shaped bacteria from antibiotic treatment, and that direct association with a tactoid is required for protection, demonstrating the formation of a diffusion barrier by the tactoid. This study provides insights into how filamentous molecules protect bacteria from extraneous substances in biofilms and in host-associated infections.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: