Small animal PET with spontaneous inhalation of 15 O-labelled oxygen gases: Longitudinal assessment of cerebral oxygen metabolism in a rat model of neonatal hypoxic-ischaemic encephalopathy.

Autor: Shimochi S; Turku PET Centre, University of Turku, Turku, Finland.; MediCity Research Laboratory, University of Turku, Turku, Finland.; Nara Institute of Science and Technology, Ikoma City, Japan., Ihalainen J; Turku PET Centre, University of Turku, Turku, Finland.; Department of Medical Physics, Turku University Hospital, Turku, Finland.; Accelerator Laboratory, Turku PET Centre, Åbo Akademi University, Turku, Finland., Parikka V; Turku PET Centre, University of Turku, Turku, Finland.; MediCity Research Laboratory, University of Turku, Turku, Finland.; Department of Pediatrics and Adolescent Medicine, Turku University Hospital, Turku, Finland.; InFLAMES Research Flagship Center, University of Turku, Turku, Finland., Kudomi N; Department of Medical Physics, Faculty of Medicine, Kagawa University, Kagawa, Japan., Tolvanen T; Turku PET Centre, University of Turku, Turku, Finland.; Department of Medical Physics, Turku University Hospital, Turku, Finland., Hietanen A; Turku PET Centre, University of Turku, Turku, Finland., Kokkomäki E; Turku PET Centre, University of Turku, Turku, Finland., Johansson S; Accelerator Laboratory, Turku PET Centre, Åbo Akademi University, Turku, Finland., Tsuji M; Department of Food and Nutrition, Kyoto Women's University, Kyoto, Japan., Kanaya S; Nara Institute of Science and Technology, Ikoma City, Japan., Yatkin E; Central Animal Laboratory, University of Turku, Turku, Finland., Grönroos TJ; Turku PET Centre, University of Turku, Turku, Finland.; MediCity Research Laboratory, University of Turku, Turku, Finland., Iida H; Turku PET Centre, University of Turku, Turku, Finland.; Nara Institute of Science and Technology, Ikoma City, Japan.
Jazyk: angličtina
Zdroj: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism [J Cereb Blood Flow Metab] 2024 Jun; Vol. 44 (6), pp. 1024-1038. Date of Electronic Publication: 2023 Dec 19.
DOI: 10.1177/0271678X231220691
Abstrakt: Perinatal hypoxic-ischaemic encephalopathy (HIE) is the leading cause of irreversible brain damage resulting in serious neurological dysfunction among neonates. We evaluated the feasibility of positron emission tomography (PET) methodology with 15 O-labelled gases without intravenous or tracheal cannulation for assessing temporal changes in cerebral blood flow ( CBF ) and cerebral metabolic rate for oxygen ( CMRO 2 ) in a neonatal HIE rat model. Sequential PET scans with spontaneous inhalation of 15 O-gases mixed with isoflurane were performed over 14 days after the hypoxic-ischaemic insult in HIE pups and age-matched controls. CBF and CMRO 2 in the injured hemispheres of HIE pups remarkably decreased 2 days after the insult, gradually recovering over 14 days in line with their increase found in healthy controls according to their natural maturation process. The magnitude of hemispheric tissue loss histologically measured after the last PET scan was significantly correlated with the decreases in CBF and CMRO 2. This fully non-invasive imaging strategy may be useful for monitoring damage progression in neonatal HIE and for evaluating potential therapeutic outcomes.
Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze: MEDLINE
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