Study of hesperetin effect on modulating transcription levels of MLH1 and MSH2 genes in SKBR3 breast cancer cell line.

Autor: Saleh NH; Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran., Al-Khafaji ASK; Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq., Babaei E; Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Jazyk: angličtina
Zdroj: Journal of advanced pharmaceutical technology & research [J Adv Pharm Technol Res] 2023 Oct-Dec; Vol. 14 (4), pp. 338-344. Date of Electronic Publication: 2023 Oct 30.
DOI: 10.4103/JAPTR.JAPTR_278_23
Abstrakt: Hesperetin (HSP), a flavonoid, has been validated to modify gene expression and function as an epigenetic agent to stop the development of breast carcinoma cells. HSP was investigated in this research to evaluate the expression of the MLH1 and MSH2 genes in cancerous breast cell lines (SKBR3) and healthy cell lines (MCF-11A) after exposure to different dosages (200, 400, and 600 µM/mL) of HSP. After 48 h of exposure, SKBR3's half-maximal inhibitory concentration was 289.6 µM/mL and MCF-10A's was 855.4 µM/mL. The research found that increasing HSP concentrations were closely correlated with an increase in MLH1 gene levels in the SKBR3 cell line, as shown by median and percentile values. HSP therapy caused the MLH1 gene expression to substantially vary in different groups, and in the SKBR3 cell line, MSH2 gene expressions were elevated in a dose-escalating manner. Moreover, HSP also raised the number of apoptotic cells, with the fraction of apoptotic cells escalating substantially at doses of 400 and 600 µM/mL. The outcomes suggested that HSP has the potential to be utilized as a therapeutic intervention for breast cancer, as it can induce apoptosis and reduce cell viability.
Competing Interests: There are no conflicts of interest.
(Copyright: © 2023 Journal of Advanced Pharmaceutical Technology & Research.)
Databáze: MEDLINE
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