Quantification of growth and nutrient consumption of bacterial and fungal cultures in microfluidic microhabitat models.
| Autor: | Arellano-Caicedo C; Center of Microbiology and Environmental System Sciences, University of Vienna, 1030 Vienna, Austria; Department of Biology, Lund University, 22362 Lund, Sweden. Electronic address: carlos.arellano@univie.ac.at., Beech JP; Division of Solid-State Physics, Lund University, 22363 Lund, Sweden., Bengtsson M; Department of Biomedical Engineering, Lund University, 22363 Lund, Sweden., Ohlsson P; Department of Biomedical Engineering, Lund University, 22363 Lund, Sweden., Hammer EC; Department of Biology, Lund University, 22362 Lund, Sweden. Electronic address: edith.hammer@biol.lu.se. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Mar 15; Vol. 5 (1), pp. 102784. Date of Electronic Publication: 2023 Dec 15. |
| DOI: | 10.1016/j.xpro.2023.102784 |
| Abstrakt: | Understanding microbes in nature requires consideration of their microenvironment. Here, we present a protocol for quantifying biomass and nutrient degradation of bacterial and fungal cultures (Pseudomonas putida and Coprinopsis cinerea, respectively) in microfluidics. We describe steps for mask design and fabrication, master printing, polydimethylsiloxane chip fabrication, and chip inoculation and imaging using fluorescence microscopy. We include procedures for image analysis, plotting, and statistics. For complete details on the use and execution of this protocol, please refer to Arellano-Caicedo et al. (2023). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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