Hypometabolism to survive the long polar night and subsequent successful return to light in the diatom Fragilariopsis cylindrus.

Autor: Joli N; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France., Concia L; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France., Mocaer K; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL) & Collaboration for Joint PhD Degree between the European Molecular Biology Laboratory and the Heidelberg University, Faculty of Biosciences, 69117, Heidelberg, Germany., Guterman J; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France., Laude J; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France., Guerin S; Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec-Océan, Université Laval, Québec, QC, G1V 0A6, Canada., Sciandra T; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France.; Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec-Océan, Université Laval, Québec, QC, G1V 0A6, Canada., Bruyant F; Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec-Océan, Université Laval, Québec, QC, G1V 0A6, Canada., Ait-Mohamed O; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France., Beguin M; Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec-Océan, Université Laval, Québec, QC, G1V 0A6, Canada., Forget MH; Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec-Océan, Université Laval, Québec, QC, G1V 0A6, Canada., Bourbousse C; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France., Lacour T; Laboratoire PHYSiologie des micro ALGues (PDG-ODE-PHYTOX-PHYSALG), Centre Atlantique, 44 311, Nantes, France., Bailleul B; Laboratory of Chloroplast Biology and Light Sensing in Microalgae, Institut de Biologie Physico Chimique, CNRS, Sorbonne Université, Paris, 75005, France., Nef C; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France., Savoie M; Département de Biologie, Université Laval, Québec, QC, G1V 0A6, Canada., Tremblay JE; Mount Allison University, Sackville, NB, E4L 1H3, Canada., Campbell DA; Département de Biologie, Université Laval, Québec, QC, G1V 0A6, Canada., Lavaud J; Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec-Océan, Université Laval, Québec, QC, G1V 0A6, Canada.; UMR 6539 LEMAR-Laboratory of Environmental Marine Sciences, CNRS/Univ Brest/Ifremer/IRD, IUEM-Institut Européen de la Mer, Technopôle Brest-Iroise, rue Dumont d'Urville, 29280, Plouzané, France., Schwab Y; Cell Biology and Biophysics Unit and Electron Microscopy Core Facility, European Molecular Biology Laboratory (EMBL), 69117, Heidelberg, Germany., Babin M; Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec-Océan, Université Laval, Québec, QC, G1V 0A6, Canada., Bowler C; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Mar; Vol. 241 (5), pp. 2193-2208. Date of Electronic Publication: 2023 Dec 14.
DOI: 10.1111/nph.19387
Abstrakt: Diatoms, the main eukaryotic phytoplankton of the polar marine regions, are essential for the maintenance of food chains specific to Arctic and Antarctic ecosystems, and are experiencing major disturbances under current climate change. As such, it is fundamental to understand the physiological mechanisms and associated molecular basis of their endurance during the long polar night. Here, using the polar diatom Fragilariopsis cylindrus, we report an integrative analysis combining transcriptomic, microscopic and biochemical approaches to shed light on the strategies used to survive the polar night. We reveal that in prolonged darkness, diatom cells enter a state of quiescence with reduced metabolic and transcriptional activity, during which no cell division occurs. We propose that minimal energy is provided by respiration and degradation of protein, carbohydrate and lipid stores and that homeostasis is maintained by autophagy in prolonged darkness. We also report internal structural changes that manifest the morphological acclimation of cells to darkness, including the appearance of a large vacuole. Our results further show that immediately following a return to light, diatom cells are able to use photoprotective mechanisms and rapidly resume photosynthesis, demonstrating the remarkable robustness of polar diatoms to prolonged darkness at low temperature.
(© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.)
Databáze: MEDLINE
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