Facile hyperpolarization chemistry for molecular imaging and metabolic tracking of [1- 13 C]pyruvate in vivo.

Autor:	MacCulloch K; Department of Chemistry, North Carolina State University, Raleigh, NC, 27695,USA., Browning A; Department of Chemistry, North Carolina State University, Raleigh, NC, 27695,USA., Bedoya DOG; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA., McBride SJ; Department of Chemistry, North Carolina State University, Raleigh, NC, 27695,USA., Abdulmojeed MB; Department of Chemistry, North Carolina State University, Raleigh, NC, 27695,USA., Dedesma C; Vizma Life Sciences Inc., Chapel Hill, NC, 27514, United States., Goodson BM; School of Chemical & Biomolecular Sciences and Materials Technology Center, Southern Illinois University, Carbondale, IL, 62901, USA., Rosen MS; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA., Chekmenev EY; Department of Chemistry, Integrative Bio-sciences (Ibio), Karmanos Cancer Institute (KCI), Wayne State University, Detroit, MI 48202, USA.; Russian Academy of Sciences, 119991 Moscow, Russia., Yen YF; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, USA., TomHon P; Vizma Life Sciences Inc., Chapel Hill, NC, 27514, United States., Theis T; Department of Chemistry, North Carolina State University, Raleigh, NC, 27695,USA.; Department of Physics, North Carolina State University, Raleigh, NC 27606, USA.; Joint UNC & NC State Department of Biomedical Engineering, North Carolina State University, Raleigh, NC 27606, USA.
Jazyk:	angličtina
Zdroj:	Journal of magnetic resonance open [J Magn Reson Open] 2023 Dec; Vol. 16-17. Date of Electronic Publication: 2023 Jul 13.
DOI:	10.1016/j.jmro.2023.100129
Abstrakt:	Hyperpolarization chemistry based on reversible exchange of parahydrogen, also known as Signal Amplification By Reversible Exchange (SABRE), is a particularly simple approach to attain high levels of nuclear spin hyperpolarization, which can enhance NMR and MRI signals by many orders of magnitude. SABRE has received significant attention in the scientific community since its inception because of its relative experimental simplicity and its broad applicability to a wide range of molecules, however in vivo detection of molecular probes hyperpolarized by SABRE has remained elusive. Here we describe a first demonstration of SABRE-hyperpolarized contrast detected in vivo, specifically using hyperpolarized [1- 13 C]pyruvate. Biocompatible formulations of hyperpolarized [1- 13 C]pyruvate in, both, methanol-water mixtures, and ethanol-water mixtures followed by dilution with saline and catalyst filtration were prepared and injected into healthy Sprague Dawley and Wistar rats. Effective hyperpolarization-catalyst removal was performed with silica filters without major losses in hyperpolarization. Metabolic conversion of pyruvate to lactate, alanine, and bicarbonate was detected in vivo . Pyruvate-hydrate was also observed as minor byproduct. Measurements were performed on the liver and kidney at 4.7 T via time-resolved spectroscopy and chemical-shift-resolved MRI. In addition, whole-body metabolic measurements were obtained using a cryogen-free 1.5 T MRI system, illustrating the utility of combining lower-cost MRI systems with simple, low-cost hyperpolarization chemistry to develop safe, and scalable molecular imaging. Competing Interests: Notes, Conflict of Interest Disclosures PT, CD, TT and MSR are co-founders and equity holders of Vizma Life Sciences (VLS). CD and PT are employees of VLS. EYC is an equity holder of Vizma Life Sciences. The terms of TT's arrangement have been reviewed and approved by NC State University in accordance with its policy on objectivity in research. EYC and BMG hold ownership stakes in XeUS Technologies Ltd. MSR is a founder and equity holder of Hyperfine Inc. All other authors declare that they have no competing interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu