Spectral effects and enhancement quantification in healthy human saliva with surface-enhanced Raman spectroscopy using silver nanopillar substrates.

Autor: Zamani E; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada., Ksantini N; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada., Sheehy G; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada., Ember KJI; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada., Baloukas B; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada., Zabeida O; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada., Trang T; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada., Mahfoud M; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada., Sapieha JE; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada., Martinu L; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada., Leblond F; Department of Engineering Physics, Polytechnique Montreal, Montréal, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada.
Jazyk: angličtina
Zdroj: Lasers in surgery and medicine [Lasers Surg Med] 2024 Feb; Vol. 56 (2), pp. 206-217. Date of Electronic Publication: 2023 Dec 10.
DOI: 10.1002/lsm.23746
Abstrakt: Objectives: Raman spectroscopy as a diagnostic tool for biofluid applications is limited by low inelastic scattering contributions compared to the fluorescence background from biomolecules. Surface-enhanced Raman spectroscopy (SERS) can increase Raman scattering signals, thereby offering the potential to reduce imaging times. We aimed to evaluate the enhancement related to the plasmonic effect and quantify the improvements in terms of spectral quality associated with SERS measurements in human saliva.
Methods: Dried human saliva was characterized using spontaneous Raman spectroscopy and SERS. A fabrication protocol was implemented leading to the production of silver (Ag) nanopillar substrates by glancing angle deposition. Two different imaging systems were used to interrogate saliva from 161 healthy donors: a custom single-point macroscopic system and a Raman micro-spectroscopy instrument. Quantitative metrics were established to compare spontaneous RS and SERS measurements: the Raman spectroscopy quality factor (QF), the photonic count rate (PR), the signal-to-background ratio (SBR).
Results: SERS measurements acquired with an excitation energy four times smaller than with spontaneous RS resulted in improved QF, PR values an order of magnitude larger and a SBR twice as large. The SERS enhancement reached 100×, depending on which Raman bands were considered.
Conclusions: Single-point measurement of dried saliva with silver nanopillars substrates led to reproducible SERS measurements, paving the way to real-time tools of diagnosis in human biofluids.
(© 2023 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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