Phenotypic Analysis of Early Neurogenesis in a Mouse Chimeric Embryo and Stem Cell-Based Neuruloid Model.

Autor:	McMahon R; Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia., Masamsetti VP; Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia., Tam PPL; Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia. ptam@cmri.org.au.; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia. ptam@cmri.org.au.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2746, pp. 165-177.
DOI:	10.1007/978-1-0716-3585-8_14
Abstrakt:	Analyzing the impact of genetic mutations on early neurogenesis of mammalian embryos in conventional mouse mutant models is laborious and time-consuming. To overcome these constraints and to fast-track the phenotypic analysis, we developed a protocol that harnesses the amenability of engineering genetic modifications in embryonic stem cells from which mid-gestation mouse chimeras and in vitro neuruloids are generated. These stem cell-based chimera and neuruloid experimental models allow phenotyping at early developmental time points of neurogenesis. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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