Characterization of a novel lytic bacteriophage VPMCC14 which efficiently controls Vibrio harveyi in Penaeus monodon culture.

Autor: Kar P; Department of Biological Sciences, Midnapore City College, Midnapore, West Bengal, 721129, India.; Biodiversity and Environmental Studies Research Center, Midnapore City College affiliated to Vidyasagar University, Midnapore, West Bengal, India., Ghosh S; Department of Biological Sciences, Midnapore City College, Midnapore, West Bengal, 721129, India.; Biodiversity and Environmental Studies Research Center, Midnapore City College affiliated to Vidyasagar University, Midnapore, West Bengal, India., Payra P; Department of Industrial Fish & Fisheries, Ramnagar College, Depal, Ramnagar, West Bengal, India., Chakrabarti S; Department of Biological Sciences, Midnapore City College, Midnapore, West Bengal, 721129, India., Pradhan S; Department of Biological Sciences, Midnapore City College, Midnapore, West Bengal, 721129, India., Mondal KC; Department of Microbiology, Vidyasagar University, Midnapore, West Bengal, India., Ghosh K; Department of Biological Sciences, Midnapore City College, Midnapore, West Bengal, 721129, India. micro.kuntal@gmail.com.
Jazyk: angličtina
Zdroj: International microbiology : the official journal of the Spanish Society for Microbiology [Int Microbiol] 2024 Aug; Vol. 27 (4), pp. 1083-1093. Date of Electronic Publication: 2023 Dec 03.
DOI: 10.1007/s10123-023-00456-9
Abstrakt: Vibrio harveyi causes luminous vibriosis diseases in shrimp, which lead to shrimp mortalities. Considering the emergence of antibiotic-resistant bacteria, a Vibrio-infecting bacteriophage, VPMCC14, was characterized, and its lysis ability was evaluated on a laboratory scale. VPMCC14 was shown to infect V. harveyi S5A and V. harveyi ATCC 14126. VPMCC14 also exhibited a latent period of 30 min, with a burst size of 38 PFU/cell on its propagation strain. The bacteriophage was stable at a wide range of pHs (3-9), temperatures (0-45°C), and salinities (up to 40 ppt). VPMCC14 exhibited strict virulence properties as the bacteriophage entirely lysed V. harveyi S5A in liquid culture inhibition after 5 h and 4 h at very low MOIs such as MOI 0.1 and MOI 1, respectively. VPMCC14 could control V. harveyi infection in aquariums at MOI 1 and decrease the mortality of Penaeus monodon challenged by V. harveyi. VPMCC14 genome was 134,472 bp long with a 34.5 G+C% content, and 240 open reading frames. A unique characteristic of VPMCC14 was the presence of the HicB family antitoxin-coding open reading frame. Comparative genomic analyses suggested that VPMCC14 could be a representative of a new genus in the Caudoviricetes class. This novel bacteriophage, VPMCC14, could be applied as a biocontrol agent for controlling V. harveyi infection.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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