Multiple SARS-CoV-2 immunizations of an unvaccinated population lead to complex immunity. A T cell reactivity study of blood donors in Antananarivo.

Autor:	Razafimahatratra SL; Immunology of Infectious Diseases Unit, Institut Pasteur of Madagascar, 101 Antananarivo, Madagascar., Andriatefy OH; Immunology of Infectious Diseases Unit, Institut Pasteur of Madagascar, 101 Antananarivo, Madagascar., Mioramalala DJN; Immunology of Infectious Diseases Unit, Institut Pasteur of Madagascar, 101 Antananarivo, Madagascar., Tsatoromila FAM; Regional Blood Transfusion Centre of Analamanga, 101 Antananarivo, Madagascar., Randrianarisaona F; Immunology of Infectious Diseases Unit, Institut Pasteur of Madagascar, 101 Antananarivo, Madagascar., Dussart P; Direction, Institut Pasteur of Madagascar, 101 Antananarivo, Madagascar., Schoenhals M; Immunology of Infectious Diseases Unit, Institut Pasteur of Madagascar, 101 Antananarivo, Madagascar. Electronic address: schoenhals@pasteur.mg.
Jazyk:	angličtina
Zdroj:	Journal of infection and public health [J Infect Public Health] 2024 Jan; Vol. 17 (1), pp. 175-181. Date of Electronic Publication: 2023 Nov 22.
DOI:	10.1016/j.jiph.2023.11.020
Abstrakt:	Background: Madagascar has undergone multiple and robust COVID-19 waves. The resulting immune background developed by its poorly vaccinated population has however not been described. Methods: In this study, serological analysis and specific T cell response descriptions were used to describe the history of exposures of the capital's blood donors to SARS-CoV-2 and its VOCs. Samples were collected early 2022, and pools of multiple immunogenic peptides of SARS-CoV-2 were used in an IFN-γ secretion ELISPOT assay to characterize the specific T-cell immunity developed against these potential epitopes. Results: Multiple epidemic waves have led to 92.1% of donors having detectable antibodies, and 94.8% having developed T-cells against SARS-CoV-2. Heterogeneous reactivities to different strain-derived peptides suggested multiple immunological backgrounds in the population including 16.1% of individuals exposed at least once to a unique strain, 27.1% to two strains, 28.5% to three strains, and 23.1% to four distinct strains. Conclusions: Cross-reactivity increased with multiple exposures but did not decrease the risk of re-infection. These results describe the extremely complex immunological background developed following multiple natural immunizations. Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interest. (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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