Transcriptomic signature of luteinized cumulus cells of oocytes developing to live birth after women received intracytoplasmic sperm injection.

Autor:	Sachs MK; Department of Reproductive Endocrinology, University Hospital Zurich, Frauenklinikstrasse, Zurich, Switzerland. Electronic address: maike.sachs@usz.ch., Makieva S; Department of Reproductive Endocrinology, University Hospital Zurich, Frauenklinikstrasse, Zurich, Switzerland., Velasco Gil A; Department of Reproductive Endocrinology, University Hospital Zurich, Frauenklinikstrasse, Zurich, Switzerland., Xie M; Department of Reproductive Endocrinology, University Hospital Zurich, Frauenklinikstrasse, Zurich, Switzerland., Ille F; Center of Bioscience and Medical Engineering, Institute of Medical Engineering, Lucerne University of Applied Sciences and Arts, Obermattweg, Hergiswil, Switzerland., Salvadori V; Center of Bioscience and Medical Engineering, Institute of Medical Engineering, Lucerne University of Applied Sciences and Arts, Obermattweg, Hergiswil, Switzerland., Schmidhauser M; ETH Zürich, Animal Physiology, Institute of Agricultural Sciences, Universitätsstrasse Zurich, Switzerland., Saenz-de-Juano MD; ETH Zürich, Animal Physiology, Institute of Agricultural Sciences, Universitätsstrasse Zurich, Switzerland., Ulbrich SE; ETH Zürich, Animal Physiology, Institute of Agricultural Sciences, Universitätsstrasse Zurich, Switzerland., Leeners B; Department of Reproductive Endocrinology, University Hospital Zurich, Frauenklinikstrasse, Zurich, Switzerland.
Jazyk:	angličtina
Zdroj:	F&S science [F S Sci] 2024 Feb; Vol. 5 (1), pp. 24-38. Date of Electronic Publication: 2023 Nov 28.
DOI:	10.1016/j.xfss.2023.11.004
Abstrakt:	Objective: To compare the transcriptome of human cumulus cells (CCs) from oocytes with different outcomes (pregnancy yes/no, live birth [LB] yes/no), to identify noninvasive biomarkers for oocyte selection as well as new therapeutic targets to increase LB rates from assisted reproductive technologies (ART). Design: Retrospective observational study. Settings: This study was conducted at a University Hospital in Switzerland. Patients: Subfertile couples undergoing controlled ovarian superstimulation and intracytoplasmic sperm injection with subsequent unbiopsied embryo transfer below the female age of 43 years. Intervention(s): RNA sequencing of CCs from oocytes results in a pregnancy, no pregnancy, LB, or no LB. Main Outcome Measures: Differential gene expression (DEG) between CCs of oocytes results in "no pregnancy" vs. "pregnancy" and "pregnancy only" vs. "live birth." Results: Although RNA sequencing did not reveal DEGs when comparing the transcriptomic profiles of the groups "no pregnancy" with "pregnancy," we identified 139 DEGs by comparing "pregnancy only" with "live birth," of which 28 belonged to clusters relevant to successful ART outcomes (i.e., CTGF, SERPINE2, PCK1, HHIP, HS3ST, and BIRC5). A functional enrichment analysis revealed that the transcriptome of CCs associated with LB depicts pathways of extracellular matrix, inflammatory cascades leading to ovulation, cell patterning, proliferation, and differentiation, and silencing pathways leading to apoptosis. Conclusion: We identified a CCs transcriptomic profile associated with LB after embryo transfer that, after further validation, could serve to predict successful ART outcomes. The definition of relevant pathways of CCs related to oocyte competency contributes to a broader understanding of the cumulus oocyte complex and helps identify further therapeutic targets for improving ART success. Competing Interests: Declaration of interests M.K.S. reports funding from SGGG-Bayer Grant for the submitted work; travel support from IBSA for ESHRE conference outside the submitted work. S.M. has nothing to disclose. A.V.G. has nothing to disclose. M.X. has nothing to disclose. F.I. has nothing to disclose. V.S. has nothing to disclose. M.S. has nothing to disclose. M.D.S. has nothing to disclose. S.E.U. has nothing to disclose. B.L. reports funding from University Research Priority Program “Human Reproduction Reloaded for the submitted work; travel support from IBSA: Support to attend ESHRE, ASRM; leadership roles Swiss Society of Reproductive Medicine (SGRM) Arbeitsgemeinschaft für Gynäkologische Endokrinologie und Reproduktionsmedizin (AGER); stock options from Alife outside the submitted work. (Copyright © 2023 American Society for Reproductive Medicine. All rights reserved.)
Databáze:	MEDLINE
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