Cellular development and evolution of the mammalian cerebellum.

Autor: Sepp M; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany. m.sepp@zmbh.uni-heidelberg.de., Leiss K; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany. k.leiss@zmbh.uni-heidelberg.de., Murat F; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.; INRAE, LPGP, Rennes, France., Okonechnikov K; Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.; Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany., Joshi P; Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.; Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany.; Developmental Origins of Pediatric Cancer Junior Group, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany., Leushkin E; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany., Spänig L; Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.; Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany.; Developmental Origins of Pediatric Cancer Junior Group, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany., Mbengue N; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany., Schneider C; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany., Schmidt J; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany., Trost N; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany., Schauer M; Museum für Naturkunde Berlin, Leibniz Institute for Evolution and Biodiversity Science, Berlin, Germany., Khaitovich P; NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China., Lisgo S; Biosciences Institute, Newcastle University, Newcastle, UK., Palkovits M; Human Brain Tissue Bank, Semmelweis University, Budapest, Hungary., Giere P; Museum für Naturkunde Berlin, Leibniz Institute for Evolution and Biodiversity Science, Berlin, Germany., Kutscher LM; Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.; Developmental Origins of Pediatric Cancer Junior Group, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany., Anders S; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.; BioQuant, Heidelberg University, Heidelberg, Germany., Cardoso-Moreira M; Evolutionary Developmental Biology Laboratory, Francis Crick Institute, London, UK., Sarropoulos I; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany. i.sarropoulos@zmbh.uni-heidelberg.de.; Wellcome Sanger Institute, Cambridge, UK. i.sarropoulos@zmbh.uni-heidelberg.de., Pfister SM; Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany. s.pfister@kitz-heidelberg.de.; Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany. s.pfister@kitz-heidelberg.de.; Department of Pediatric Hematology and Oncology, Heidelberg University Hospital, Heidelberg, Germany. s.pfister@kitz-heidelberg.de.; National Center for Tumor Diseases (NCT), Heidelberg, Germany. s.pfister@kitz-heidelberg.de., Kaessmann H; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany. h.kaessmann@zmbh.uni-heidelberg.de.
Jazyk: angličtina
Zdroj: Nature [Nature] 2024 Jan; Vol. 625 (7996), pp. 788-796. Date of Electronic Publication: 2023 Nov 29.
DOI: 10.1038/s41586-023-06884-x
Abstrakt: The expansion of the neocortex, a hallmark of mammalian evolution 1,2 , was accompanied by an increase in cerebellar neuron numbers 3 . However, little is known about the evolution of the cellular programmes underlying the development of the cerebellum in mammals. In this study we generated single-nucleus RNA-sequencing data for around 400,000 cells to trace the development of the cerebellum from early neurogenesis to adulthood in human, mouse and the marsupial opossum. We established a consensus classification of the cellular diversity in the developing mammalian cerebellum and validated it by spatial mapping in the fetal human cerebellum. Our cross-species analyses revealed largely conserved developmental dynamics of cell-type generation, except for Purkinje cells, for which we observed an expansion of early-born subtypes in the human lineage. Global transcriptome profiles, conserved cell-state markers and gene-expression trajectories across neuronal differentiation show that cerebellar cell-type-defining programmes have been overall preserved for at least 160 million years. However, we also identified many orthologous genes that gained or lost expression in cerebellar neural cell types in one of the species or evolved new expression trajectories during neuronal differentiation, indicating widespread gene repurposing at the cell-type level. In sum, our study unveils shared and lineage-specific gene-expression programmes governing the development of cerebellar cells and expands our understanding of mammalian brain evolution.
(© 2023. The Author(s).)
Databáze: MEDLINE
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