CRISPR-Cas9-mediated IncF plasmid curing in extraintestinal pathogenic Escherichia coli .
| Autor: | Chen L; Center for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.; Hackensack Meridian School of Medicine , Nutley, New Jersey, USA., Peirano G; Cummings School of Medicine, University of Calgary , Calgary, Alberta, Canada.; Alberta Precision Laboratories , Calgary, Alberta, Canada., Yen K; Center for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA., Wang B; Center for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.; Department of Clinical Laboratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine , Shanghai, China., Terlecky A; Center for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA., DeVinney R; Cummings School of Medicine, University of Calgary , Calgary, Alberta, Canada., Kreiswirth BN; Center for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.; Hackensack Meridian School of Medicine , Nutley, New Jersey, USA., Pitout JDD; Cummings School of Medicine, University of Calgary , Calgary, Alberta, Canada.; Alberta Precision Laboratories , Calgary, Alberta, Canada.; University of Pretoria , Pretoria, Gauteng, South Africa. |
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| Jazyk: | angličtina |
| Zdroj: | Microbiology spectrum [Microbiol Spectr] 2024 Jan 11; Vol. 12 (1), pp. e0369223. Date of Electronic Publication: 2023 Nov 29. |
| DOI: | 10.1128/spectrum.03692-23 |
| Abstrakt: | Importance: Understanding the role of IncF plasmids in the success of drug-resistant bacteria has far-reaching implications for tackling antibiotic resistance. The study's use of a novel CRISPR-Cas9-mediated plasmid-curing system provides a precision tool for dissecting the specific impact of IncF plasmids on ExPEC clones, especially high-risk, multidrug-resistant strains like ST131, ST1193, and ST410. The study offers a crucial stepping stone for future research into understanding how these plasmids influence more complex aspects of bacterial behavior, such as cell invasion and in vivo fitness. Competing Interests: The authors declare no conflict of interest. |
| Databáze: | MEDLINE |
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