| Autor: |
Yang Y; Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.; Xinjiang Academy of Agricultural Sciences Kuqa County Upland Cotton Test Station, Xinjiang Academy of Agricultural Sciences, Kuqa 842000, China., Sun F; State Key Laboratory of Cotton Biology, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang 455000, China., Wang P; Xinjiang Academy of Agricultural Sciences Kuqa County Upland Cotton Test Station, Xinjiang Academy of Agricultural Sciences, Kuqa 842000, China., Yusuyin M; Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China., Kuerban W; Xinjiang Academy of Agricultural Sciences Kuqa County Upland Cotton Test Station, Xinjiang Academy of Agricultural Sciences, Kuqa 842000, China., Lai C; Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China., Li C; Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China., Ma J; Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China., Xiao F; Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. |
| Abstrakt: |
The β-amylase ( BAM ) gene family encodes important enzymes that catalyze the conversion of starch to maltose in various biological processes of plants and play essential roles in regulating the growth and development of multiple plants. So far, BAMs have been extensively studied in Arabidopsis thaliana ( A. thaliana ). However, the characteristics of the BAM gene family in the crucial economic crop, cotton, have not been reported. In this study, 27 GhBAM genes in the genome of Gossypium hirsutum L ( G. hirsutum ) were identified by genome-wide identification, and they were divided into three groups according to sequence similarity and phylogenetic relationship. The gene structure, chromosome distribution, and collinearity of all GhBAM genes identified in the genome of G. hirsutum were analyzed. Further sequence alignment of the core domain of glucosyl hydrolase showed that all GhBAM family genes had the glycosyl hydrolase family 14 domain. We identified the BAM gene GhBAM7 and preliminarily investigated its function by transcriptional sequencing analysis, qRT-PCR, and subcellular localization. These results suggested that the GhBAM7 gene may influence fiber strength during fiber development. This systematic analysis provides new insight into the transcriptional characteristics of BAM genes in G. hirsutum . It may lay the foundation for further study of the function of these genes. |
