HIV-1 Gag co-localizes with euchromatin histone marks at the nuclear periphery.
| Autor: | Chang J; Department of Medicine, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA., Parent LJ; Department of Medicine, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.; Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of virology [J Virol] 2023 Dec 21; Vol. 97 (12), pp. e0117923. Date of Electronic Publication: 2023 Nov 22. |
| DOI: | 10.1128/jvi.01179-23 |
| Abstrakt: | Importance: The traditional view of retrovirus assembly posits that packaging of gRNA by HIV-1 Gag occurs in the cytoplasm or at the plasma membrane. However, our previous studies showing that HIV-1 Gag enters the nucleus and binds to USvRNA at transcription sites suggest that gRNA selection may occur in the nucleus. In the present study, we observed that HIV-1 Gag trafficked to the nucleus and co-localized with USvRNA within 8 hours of expression. In infected T cells (J-Lat 10.6) reactivated from latency and in a HeLa cell line stably expressing an inducible Rev-dependent HIV-1 construct, we found that Gag preferentially localized with euchromatin histone marks associated with enhancer and promoter regions near the nuclear periphery, which is the favored site HIV-1 integration. These observations support the innovative hypothesis that HIV-1 Gag associates with euchromatin-associated histones to localize to active transcription sites, promoting capture of newly synthesized gRNA for packaging. Competing Interests: The authors declare no conflict of interest. |
| Databáze: | MEDLINE |
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