Evaluation of lateral flow devices for postmortem rabies diagnosis in animals in the Philippines: a multicenter study.

Autor:	Cruz JL; Department of Agriculture, Bureau of Animal Industry , Quezon, Philippines., Garcia AM; Department of Microbiology, Faculty of Medicine, Oita University , Yufu, Oita, Japan., Saito N; Department of Microbiology, Faculty of Medicine, Oita University , Yufu, Oita, Japan., Lagayan MGO; Department of Agriculture, Bureau of Animal Industry , Quezon, Philippines., Dela Peña RC; Department of Agriculture, Bureau of Animal Industry , Quezon, Philippines., Usana MS; Regional Animal Disease Diagnostic Laboratory I, Sta Barbara , Pangasinan, Philippines., Agustin SP; Regional Animal Disease Diagnostic Laboratory I, Sta Barbara , Pangasinan, Philippines., Tattao JZ; Regional Animal Disease Diagnostic Laboratory II , Tuguegarao, Cagayan, Philippines., Mamauag CV; Regional Animal Disease Diagnostic Laboratory II , Tuguegarao, Cagayan, Philippines., Ducayag OP; Regional Animal Disease Diagnostic Laboratory CAR , Baguio, Benguet, Philippines., Nabus HLF; Regional Animal Disease Diagnostic Laboratory CAR , Baguio, Benguet, Philippines., Flores PDDL; Regional Animal Disease Diagnostic Laboratory IVA , Lipa, Batangas, Philippines., Fabon RJA; Regional Animal Disease Diagnostic Laboratory IVA , Lipa, Batangas, Philippines., Peñaflor RP; Regional Animal Disease Diagnostic Laboratory IVB , Naujan, Oriental Mindoro, Philippines., Viñas DCG; Regional Animal Disease Diagnostic Laboratory IVB , Naujan, Oriental Mindoro, Philippines., Limsan CA; Regional Animal Disease Diagnostic Laboratory IVB - Satellite Laboratory , Puerto Princesa, Palawan, Philippines., Bernales RP; Regional Animal Disease Diagnostic Laboratory V , Pili, Camarines Sur, Philippines., Llames MET; Regional Animal Disease Diagnostic Laboratory V , Pili, Camarines Sur, Philippines., Balopeños LE; Regional Animal Disease Diagnostic Laboratory VI , Iloilo, Philippines., Morales RG; Regional Animal Disease Diagnostic Laboratory VI , Iloilo, Philippines., Migriño AMCV; Regional Animal Disease Diagnostic Laboratory VII , Mandaue, Cebu, Philippines., Calunsag OJS; Regional Animal Disease Diagnostic Laboratory VII , Mandaue, Cebu, Philippines., Datoy JJ; Regional Animal Disease Diagnostic Laboratory IX , Zamboanga, Zamboanga del Sur, Philippines., Palma KY; Regional Animal Disease Diagnostic Laboratory IX , Zamboanga, Zamboanga del Sur, Philippines., Sepulveda MCB; Davao City Animal Disease Diagnostic Laboratory , Davao, Davao del Sur, Philippines., Eng MNJ; Davao City Animal Disease Diagnostic Laboratory , Davao, Davao del Sur, Philippines., Moscoso JS; Regional Animal Disease Diagnostic Laboratory XII , General Santos, South Cotabato, Philippines., Julabar SMF; Regional Animal Disease Diagnostic Laboratory XII , General Santos, South Cotabato, Philippines., Mauhay JD; Department of Microbiology, Faculty of Medicine, Oita University , Yufu, Oita, Japan., Espino MJM; Department of Microbiology, Faculty of Medicine, Oita University , Yufu, Oita, Japan., Javier CJM; Department of Microbiology, Faculty of Medicine, Oita University , Yufu, Oita, Japan., Kimitsuki K; Department of Microbiology, Faculty of Medicine, Oita University , Yufu, Oita, Japan., Nishizono A; Department of Microbiology, Faculty of Medicine, Oita University , Yufu, Oita, Japan.; Research Center for Global and Local Infectious Diseases, Oita University , Yufu, Oita, Japan.
Jazyk:	angličtina
Zdroj:	Journal of clinical microbiology [J Clin Microbiol] 2023 Dec 19; Vol. 61 (12), pp. e0084223. Date of Electronic Publication: 2023 Nov 22.
DOI:	10.1128/jcm.00842-23
Abstrakt:	Expansion of the use of lateral flow devices (LFD) for animal rabies diagnosis can help mitigate the widespread underreporting of rabies. However, this has been hindered by the limited number and small sample size of previous studies. To overcome this limitation, we conducted a multicenter study with a larger sample size to assess the diagnostic accuracy of the ADTEC LFD for postmortem rabies diagnosis in animals. Thirteen governmental animal diagnostic laboratories in the Philippines were involved in this study, and 791 animals suspected of having rabies were tested using both the direct fluorescence antibody test (DFAT) and ADTEC LFD between August 2021 and October 2022. The LFD demonstrated a sensitivity of 96.3% [95% confidence interval (CI): 94.1%-97.9%] and a specificity of 99.7% (95% CI: 98.4%-100%). Notably, false-negative results were more likely to occur in laboratories with lower annual processing volumes of rabies samples in the previous years (adjusted odds ratio 4.97, 95% CI: 1.49-16.53). In this multicenter study, the high sensitivity and specificity of the LFD for the diagnosis of animal rabies, compared to that of the DFAT, was demonstrated, yet concerns regarding false-negative results remain. In areas with limited experience in processing rabies samples, it is essential to provide comprehensive training and careful attention during implementation. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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