Real-time RT-PCR for Venezuelan equine encephalitis complex, Madariaga, and Eastern equine encephalitis viruses: application in human and mosquito public health surveillance in Panama.

Autor:	Carrera J-P; Department of Biology, University of Oxford , Oxford, United Kingdom.; Pandemic Sciences Institute, Nuffield Department of Medicine, University of Oxford , Oxford, United Kingdom.; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama.; Viral Emerging Disease Dynamics Group, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Araúz D; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Rojas A; Departamento de Producción, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción , San Lorenzo, Paraguay., Cardozo F; Departamento de Producción, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción , San Lorenzo, Paraguay., Stittleburg V; Division of Infectious Diseases, Department of Medicine, Emory University , Atlanta, Georgia, USA., Morales Claro I; Instituto de Medicina Tropical, Faculdade de Medicina da Universidade de São Paulo , São Paulo, Brazil.; MRC Centre for Global Infectious Disease Analysis (MRC-GIDA), Department of Infectious Disease Epidemiology, Imperial College London , London, United Kingdom., Galue J; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama.; Viral Emerging Disease Dynamics Group, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Lezcano-Coba C; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama.; Viral Emerging Disease Dynamics Group, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Romero Rebello Moreira F; MRC Centre for Global Infectious Disease Analysis (MRC-GIDA), Department of Infectious Disease Epidemiology, Imperial College London , London, United Kingdom.; Departamento de Genética, Universidade Federal do Rio de Janeiro , Rio de Janeiro, Brazil., -Rivera LF; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama.; Viral Emerging Disease Dynamics Group, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Chen-Germán M; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Moreno B; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Capitan-Barrios Z; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama.; Viral Emerging Disease Dynamics Group, Gorgas Memorial Institute of Health Studies , Panama City, Panama.; Departamento de Microbiología y Parasitología, Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá , Ciudad de Panamá, Panama., López-Vergès S; Department of Research in Virology and Biotechnology, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Pascale JM; Clinical of Tropical Diseases and Research Unit, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Sabino EC; Instituto de Medicina Tropical, Faculdade de Medicina da Universidade de São Paulo , São Paulo, Brazil., Valderrama A; Viral Emerging Disease Dynamics Group, Gorgas Memorial Institute of Health Studies , Panama City, Panama.; Department of Medical Entomology, Gorgas Memorial Institute of Health Studies , Panama City, Panama., Hanley KA; Department of Biology, New Mexico State University , Las Cruces, New Mexico, USA., Donnelly CA; Pandemic Sciences Institute, Nuffield Department of Medicine, University of Oxford , Oxford, United Kingdom.; MRC Centre for Global Infectious Disease Analysis (MRC-GIDA), Department of Infectious Disease Epidemiology, Imperial College London , London, United Kingdom.; Department of Statistics, University of Oxford , Oxford, United Kingdom., Vasilakis N; Department of Pathology, The University of Texas Medical Branch , Galveston, Texas, USA.; Department of Preventive Medicine and Population Health, The University of Texas Medical Branch , Galveston, Texas, USA.; Center for Biodefense and Emerging Infectious Diseases, The University of Texas Medical Branch , Galveston, Texas, USA.; Center for Vector-Borne and Zoonotic Diseases, The University of Texas Medical Branch , Galveston, Texas, USA.; Center for Tropical Diseases, The University of Texas Medical Branch , Galveston, Texas, USA.; Institute for Human Infection and Immunity, The University of Texas Medical Branch , Galveston, Texas, USA., Faria NR; Department of Biology, University of Oxford , Oxford, United Kingdom.; Instituto de Medicina Tropical, Faculdade de Medicina da Universidade de São Paulo , São Paulo, Brazil.; MRC Centre for Global Infectious Disease Analysis (MRC-GIDA), Department of Infectious Disease Epidemiology, Imperial College London , London, United Kingdom., Waggoner JJ; Division of Infectious Diseases, Department of Medicine, Emory University , Atlanta, Georgia, USA.; Department of Global Health, Rollins School of Public Health, Emory University , Atlanta, Georgia, USA.
Jazyk:	angličtina
Zdroj:	Journal of clinical microbiology [J Clin Microbiol] 2023 Dec 19; Vol. 61 (12), pp. e0015223. Date of Electronic Publication: 2023 Nov 20.
DOI:	10.1128/jcm.00152-23
Abstrakt:	Eastern equine encephalitis virus (EEEV), Madariaga virus (MADV), and Venezuelan equine encephalitis virus complex (VEEV) are New World alphaviruses transmitted by mosquitoes. They cause febrile and sometimes severe neurological diseases in human and equine hosts. Detecting them during the acute phase is hindered by non-specific symptoms and limited diagnostic tools. We designed and clinically assessed real-time reverse transcription polymerase chain reaction assays (rRT-PCRs) for VEEV complex, MADV, and EEEV using whole-genome sequences. Validation involved 15 retrospective serum samples from 2015 to 2017 outbreaks, 150 mosquito pools from 2015, and 118 prospective samples from 2021 to 2022 surveillance in Panama. The rRT-PCRs detected VEEV complex RNA in 10 samples (66.7%) from outbreaks, with one having both VEEV complex and MADV RNAs. VEEV complex RNA was found in five suspected dengue cases from disease surveillance. The rRT-PCR assays identified VEEV complex RNA in three Culex ( Melanoconion ) vomerifer pools, leading to VEEV isolates in two. Phylogenetic analysis revealed the VEEV ID subtype in positive samples. Notably, 11.9% of dengue-like disease patients showed VEEV infections. Together, our rRT-PCR validation in human and mosquito samples suggests that this method can be incorporated into mosquito and human encephalitic alphavirus surveillance programs in endemic regions. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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