Beta-lactam, aminoglycoside, and quinolone resistance in Escherichia coli strains isolated from shrimps and mussels in the Marmara Sea.

Autor: Celik B; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye., Ergul B; Ambarli Veterinary Border Control Point Directorate, Republic of Turkiye Ministry of Agriculture and Forestry, Istanbul, Turkiye., Kekec AI; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye., Hala B; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye., Maslak B; Institute of Graduate Studies, Istanbul University - Cerrahpasa, Avcilar/Istanbul, Turkiye., Sigirci BD; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye., Kahraman BB; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye., Bagcigil AF; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye., Metiner K; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye., Ak S; Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye.
Jazyk: angličtina
Zdroj: Veterinarni medicina [Vet Med (Praha)] 2023 May 29; Vol. 68 (5), pp. 208-217. Date of Electronic Publication: 2023 May 29 (Print Publication: 2023).
DOI: 10.17221/105/2022-VETMED
Abstrakt: The purpose of the study was to examine the prevalence of  Escherichia coli in shrimps and mussels, and to determine the distribution of β-lactam, aminoglycoside, quinolone, and multi-drug resistance phenotypically and genotypically in  E. coli isolates obtained from mussels and shrimps in Istanbul. Faecal samples were collected from mussels ( n  = 96) and shrimps ( n  = 96) from the Marmara Sea coastline and fish markets in Istanbul. For the detection of antibiotic susceptibilities, seven antibiotic groups were used. β-lactamase, aminoglycoside, and quinolone genes were also determined. A total of 34 (17.7%, 15 shrimps, and 19 mussels) E. coli were isolated, and 17 (50%) were found to be resistant to one or more antimicrobials. The highest resistance was seen against aminoglycosides with 11 isolates (32.35%), followed by quinolones with 10 isolates (29.41%) and extended-spectrum β-lactamase (ESBL) with 4 isolates (11.76%). Multi-drug resistance was detected in 5 isolates (14.7%) from 3 shrimp and 2 mussel samples. The prevalence of ESBL genes was demonstrated at 3.84% in mussels and shrimp samples. There were no AmpC and carbapenemase-producing genes. These samples harbored bla CTX-M-1 ( n  = 3) and bla TEM ( n  = 4). Ten isolates were resistant to aminoglycosides genotypically. Resistance genes detected were strB in 2 isolates, aadA in 5, strB and aadA together in 3, ANT('')-Ia, aphA1 and aphA2 simultaneously in 3, aphA1 in 1, aac(3)-IIa in 1 isolate. aac(6')-Ib-cr gene was detected in only one of 10 phenotypically resistant isolates to quinolones.
Competing Interests: The authors declare no conflict of interest.
(Copyright: © 2023 Celik et al.)
Databáze: MEDLINE
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