Noninvasive imaging of rat-derived microglia and its reactivity to inflammatory molecules via acoustic impedance microscopy.

Autor: Lee CLM; Department of Applied Chemistry and Life Science, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan. christine.lee.li.mei.oc@tut.jp., Yap PS; Department of Applied Chemistry and Life Science, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan., Umemura K; Department of Applied Chemistry and Life Science, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan., Shintani T; Department of Electrical and Electronic Information Engineering, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan., Kobayashi K; Honda Electronics Co., Ltd., Toyohashi, Aichi, 441-3147, Japan., Hozumi N; Department of Electrical and Electronic Information Engineering, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan., Yoshida S; Department of Applied Chemistry and Life Science, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan.
Jazyk: angličtina
Zdroj: Journal of medical ultrasonics (2001) [J Med Ultrason (2001)] 2024 Jan; Vol. 51 (1), pp. 29-37. Date of Electronic Publication: 2023 Nov 16.
DOI: 10.1007/s10396-023-01379-8
Abstrakt: Purpose: Microglia, the brain's immune cells, play important roles in neuronal differentiation, survival, and death. The function of microglia is deeply related to the morphologies; however, it is too complex to observe conventionally and identify the condition of living microglia using optical microscopes. Herein, we proposed a new method to observe living cultured microglia and their reactivity to inflammation via the acoustic impedance mode of a scanning acoustic microscope.
Methods: Primary cultured microglia collected from rat pups exposed to acetamiprid, an insecticide, in utero were observed with both acoustic interface impedance mode (C-mode) and transparent three-dimensional impedance mode (B-mode).
Results: We characterized microglia into four types based on the results obtained from acoustic impedance, cytoskeletal information, and laser confocal imaging. Biphasic acoustic observation using B-mode and C-mode gave us information regarding the dynamic morphologies of living microglia treated with adenosine triphosphate (ATP) (600 μmol/L), which reflects distress signals from inflamed neurons. Acetamiprid exposure induced microglia response even in the neonatal period. ATP stimulus altered the shape and thickness of microglia with a change in the bulk modulus of the cell. Three-dimensional alteration with ATP stimulus could be observed only after biphasic acoustic observation using B-mode and C-mode. This acoustic observation was consistent with confocal observation using anti-Iba-1 and P2Y12 immunocytochemistry.
Conclusion: This study demonstrated the adequacy of using a scanning acoustic microscope in analyzing microglia's shape, motility, and response to inflammation.
(© 2023. The Author(s).)
Databáze: MEDLINE
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