A Guideline for Assessment and Characterization of Bacterial Biofilm Formation in the Presence of Inhibitory Compounds.
| Autor: | Elgamoudi BA; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Korolik V; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2023 Nov 05; Vol. 13 (21), pp. e4866. Date of Electronic Publication: 2023 Nov 05 (Print Publication: 2023). |
| DOI: | 10.21769/BioProtoc.4866 |
| Abstrakt: | Campylobacter jejuni, a zoonotic foodborne pathogen, is the worldwide leading cause of acute human bacterial gastroenteritis. Biofilms are a significant reservoir for survival and transmission of this pathogen, contributing to its overall antimicrobial resistance. Natural compounds such as essential oils, phytochemicals, polyphenolic extracts, and D-amino acids have been shown to have the potential to control biofilms formed by bacteria, including Campylobacter spp. This work presents a proposed guideline for assessing and characterizing bacterial biofilm formation in the presence of naturally occurring inhibitory molecules using C. jejuni as a model. The following protocols describe: i) biofilm formation inhibition assay, designed to assess the ability of naturally occurring molecules to inhibit the formation of biofilms; ii) biofilm dispersal assay, to assess the ability of naturally occurring inhibitory molecules to eradicate established biofilms; iii) confocal laser scanning microscopy (CLSM), to evaluate bacterial viability in biofilms after treatment with naturally occurring inhibitory molecules and to study the structured appearance (or architecture) of biofilm before and after treatment. Competing Interests: Competing interestsThe authors declare no conflicts of interest. (©Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license.) |
| Databáze: | MEDLINE |
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