Jumbo phages are active against extensively drug-resistant eyedrop-associated Pseudomonas aeruginosa infections.

Autor:	Cobián Güemes AG; Department of Pathology, University of California San Diego, La Jolla, California, USA., Ghatbale P; Department of Pathology, University of California San Diego, La Jolla, California, USA., Blanc AN; Department of Pathology, University of California San Diego, La Jolla, California, USA., Morgan CJ; Department of Biology, University of California San Diego, La Jolla, California, USA., Garcia A; Department of Pathology, University of California San Diego, La Jolla, California, USA., Leonard J; Department of Pathology, University of California San Diego, La Jolla, California, USA., Huang L; Department of Pathology, University of California San Diego, La Jolla, California, USA., Kovalick G; Department of Pathology, University of California San Diego, La Jolla, California, USA., Proost M; Department of Pathology, University of California San Diego, La Jolla, California, USA., Chiu M; Department of Pathology, University of California San Diego, La Jolla, California, USA., Kuo P; Department of Pathology, University of California San Diego, La Jolla, California, USA., Oh J; Department of Pathology, University of California San Diego, La Jolla, California, USA., Karthikeyan S; Department of Environmental Science and Engineering, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, California, USA.; Department of Pediatrics, University of California San Diego, La Jolla, California, USA., Knight R; Department of Pediatrics, University of California San Diego, La Jolla, California, USA.; Center for Microbiome Innovation, University of California San Diego, La Jolla, California, USA.; Department of Bioengineering, University of California San Diego, La Jolla, California, USA.; Department of Computer Sciences & Engineering, University of California San Diego, La Jolla, California, USA., Pogliano J; Department of Biology, University of California San Diego, La Jolla, California, USA.; Howard Hughes Medical Institute, University of California San Diego, La Jolla, California, USA., Schooley RT; Department of Medicine, University of California San Diego, La Jolla, California, USA., Pride DT; Department of Pathology, University of California San Diego, La Jolla, California, USA.; Howard Hughes Medical Institute, University of California San Diego, La Jolla, California, USA.; Department of Medicine, University of California San Diego, La Jolla, California, USA.
Jazyk:	angličtina
Zdroj:	Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2023 Dec 14; Vol. 67 (12), pp. e0065423. Date of Electronic Publication: 2023 Nov 06.
DOI:	10.1128/aac.00654-23
Abstrakt:	Antibiotic-resistant bacteria present an emerging challenge to human health. Their prevalence has been increasing across the globe due in part to the liberal use of antibiotics that has pressured them to develop resistance. Those bacteria that acquire mobile genetic elements are especially concerning because those plasmids may be shared readily with other microbes that can then also become antibiotic resistant. Serious infections have recently been related to the contamination of preservative-free eyedrops with extensively drug-resistant (XDR) isolates of Pseudomonas aeruginosa , already resulting in three deaths. These drug-resistant isolates cannot be managed with most conventional antibiotics. We sought to identify alternatives to conventional antibiotics for the lysis of these XDR isolates and identified multiple bacteriophages (viruses that attack bacteria) that killed them efficiently. We found both jumbo phages (>200 kb in genome size) and non-jumbo phages that were active against these isolates, the former killing more efficiently. Jumbo phages effectively killed the three separate XDR P. aeruginosa isolates both on solid and liquid medium. Given the ongoing nature of the XDR P. aeruginosa eyedrop outbreak, the identification of phages active against them provides physicians with several novel potential alternatives for treatment. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu