Species diversity, barcode, detection of pathogens and blood meal pattern in Phlebotominae (Diptera: Psychodidae) from northeastern Mexico.

Autor: Rodríguez-Rojas JJ; Facultad de Medicina, Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León 64460, Mexico., Lozano-Sardaneta YN; Facultad de Medicina, Centro de Medicina Tropical, Unidad de Medicina Experimental, Universidad Nacional Autónoma de México, Ciudad de México 06726, Mexico; Universidad Nacional Autónoma de México, Instituto de Biología, Colección Nacional de Insectos, Ciudad de México 06720, Mexico. Electronic address: nisei_sardaneta@ciencias.unam.mx., Fernández-Salas I; Facultad de Ciencias Biológicas, Laboratorio de Entomología Médica, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León 66455, Mexico., Sánchez-Casas RM; Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, General Escobedo, Nuevo León, 66054, Mexico., Becker I; Facultad de Medicina, Centro de Medicina Tropical, Unidad de Medicina Experimental, Universidad Nacional Autónoma de México, Ciudad de México 06726, Mexico. Electronic address: becker@unam.mx.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2024 Jan; Vol. 249, pp. 107064. Date of Electronic Publication: 2023 Nov 04.
DOI: 10.1016/j.actatropica.2023.107064
Abstrakt: More than 90 species of phlebotomines are vectors of parasites, bacteria, and viruses, which cause disease in animals and humans. Therefore, their study is necessary to establish prevention and control strategies. Mexico is an endemic country for leishmaniasis, mostly in the center and southern regions of the country, yet only few studies have been conducted in the northern part of the country. The present study aims to: (a) assess the alpha diversity of Phlebotominae in an annual cycle, (b) to correlate climatic variables with abundance, (c) to generate barcodes of these insects as part of the integrative taxonomy, and (d) to detect Leishmania, Wolbachia and blood sources in an area close to where a case of autochthonous leishmaniasis has been detected in Nuevo Leon, Mexico. A systematic sampling was conducted during three consecutive nights from 17:00 to 22:00 h., placing Shannon traps, CDC traps with incandescent light, and BG Sentinel 2 + BG Lure traps. A total catch effort of 660 nights/traps/hours was achieved, in which a total number of 707 phlebotomines (58% female and 42% male) of six species were collected and identified. The most abundant species were Psathyromyia cratifer (57%) and Psathyromyia shannoni sensu stricto (26%). The highest abundance (72%; 507/707) was collected during March, April and May 2021. Barcodes were generated for four species of phlebotomines, which represent new records for Mexico. For the molecular detection of microorganisms, 302 specimens were analyzed, although no specimens were positive for Leishmania spp. Wolbachia strains were detected in phlebotomines with an infection rate of 1.32% (4/302) and found in Pa. cratifer and Lu. cruciata. Likewise, human DNA was identified in female Lu. cruciata and Pa. cratifer phlebotomines. These findings indicate the presence of potential vector species of the parasite Leishmania spp. This result shows the need for further entomological surveillance to elucidate the transmission mechanisms in these northern areas of the country.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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