Antioxidants green tea extract and nordihydroguaiaretic acid confer species and strain-specific lifespan and health effects in Caenorhabditis nematodes.

Autor: Banse SA; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Sedore CA; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Johnson E; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Coleman-Hulbert AL; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Onken B; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA., Hall D; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Jackson EG; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Huynh P; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA., Foulger AC; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Guo S; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA., Garrett T; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Xue J; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA., Inman D; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Morshead ML; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Plummer WT; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Chen E; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA., Bhaumik D; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Chen MK; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Harinath G; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA., Chamoli M; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Quinn RP; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Falkowski R; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA., Edgar D; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Schmidt MO; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA., Lucanic M; The Buck Institute for Research On Aging, Novato, CA, 94945, USA., Guo M; Division of Aging Biology, National Institute On Aging, Bethesda, MD, 20892-9205, USA., Driscoll M; Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers University, Piscataway, NJ, 08854, USA. driscoll@dls.rutgers.edu., Lithgow GJ; The Buck Institute for Research On Aging, Novato, CA, 94945, USA. glithgow@buckinstitute.org., Phillips PC; Institute of Ecology and Evolution, University of Oregon, Eugene, OR, 97403, USA. pphil@uoregon.edu.
Jazyk: angličtina
Zdroj: GeroScience [Geroscience] 2024 Apr; Vol. 46 (2), pp. 2239-2251. Date of Electronic Publication: 2023 Nov 04.
DOI: 10.1007/s11357-023-00978-0
Abstrakt: The Caenorhabditis Intervention Testing Program (CITP) is an NIH-funded research consortium of investigators who conduct analyses at three independent sites to identify chemical interventions that reproducibly promote health and lifespan in a robust manner. The founding principle of the CITP is that compounds with positive effects across a genetically diverse panel of Caenorhabditis species and strains are likely engaging conserved biochemical pathways to exert their effects. As such, interventions that are broadly efficacious might be considered prominent compounds for translation for pre-clinical research and human clinical applications. Here, we report results generated using a recently streamlined pipeline approach for the evaluation of the effects of chemical compounds on lifespan and health. We studied five compounds previously shown to extend C. elegans lifespan or thought to promote mammalian health: 17α-estradiol, acarbose, green tea extract, nordihydroguaiaretic acid, and rapamycin. We found that green tea extract and nordihydroguaiaretic acid extend Caenorhabditis lifespan in a species-specific manner. Additionally, these two antioxidants conferred assay-specific effects in some studies-for example, decreasing survival for certain genetic backgrounds in manual survival assays in contrast with extended lifespan as assayed using automated C. elegans Lifespan Machines. We also observed that GTE and NDGA impact on older adult mobility capacity is dependent on genetic background, and that GTE reduces oxidative stress resistance in some Caenorhabditis strains. Overall, our analysis of the five compounds supports the general idea that genetic background and assay type can influence lifespan and health effects of compounds, and underscores that lifespan and health can be uncoupled by chemical interventions.
(© 2023. The Author(s).)
Databáze: MEDLINE
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