| Autor: |
Shaala AY, Dhaliwal HS, Bishop S, Ling NR |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1979; Vol. 27 (2), pp. 175-87. |
| DOI: |
10.1016/0022-1759(79)90263-1 |
| Abstrakt: |
Procion-dyed yeasts which have been incubated in fresh serum and washed are readily ingested by human blood monocytes and tumour macrophages during a 30 min incubation period. Uptake is enhanced by centrifugation. Intracellular yeasts can be readily distinquished from extracellular by their much slower uptake of toluidine blue. Yeast ingestion is a much more reliable test for blood monocytes than the latex bead test and it is easier to read. The ingestion test may be combined with a rosette test for surface immunoglobulins (SmIg). Since the yeasts take up immunoglobulins from human serum during the complement-coating stage it is necessary, in a combined ingestion-SmIg test, to use fresh serum from another species (sheep) for opsonisation of the yeasts. A technique is described for reducing the number of immunoglobulin-bearing monocytes to a low level with a combined ingestion-SmIg rosette technique to detect residual immunoglobulin-bearing phagocytes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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