Oocyte rescue in-vitro maturation does not adversely affect chromosome segregation during the first meiotic division.

Autor: Esbert M; IVIRMA Global Research Alliance, IVI Barcelona, Barcelona, Spain. Electronic address: marga.esbert@ivirma.com., García C; IVIRMA Global Research Alliance, IVI Barcelona, Barcelona, Spain., Cutts G; Juno Genetics, Oxford Science Park, Oxford, UK., Lara-Molina E; IVIRMA Global Research Alliance, IVI Barcelona, Barcelona, Spain., Garrido N; IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigación Sanitaria La Fe, Valencia, Spain., Ballestros A; IVIRMA Global Research Alliance, IVI Barcelona, Barcelona, Spain., Scott RT Jr; IVIRMA Global Research Alliance, RMA New Jersey, NJ, USA; Department of Obstetrics and Gynecology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA., Seli E; IVIRMA Global Research Alliance, RMA New Jersey, NJ, USA; Department of obstetrics, gynecology and reproductive Sciences, Yale School of Medicine, New Haven, CT, USA., Wells D; Juno Genetics, Oxford Science Park, Oxford, UK; Nuffield Department of Women's and Reproductive Health, John Radcliffe Hospital, University of Oxford, Oxford, UK.
Jazyk: angličtina
Zdroj: Reproductive biomedicine online [Reprod Biomed Online] 2024 Jan; Vol. 48 (1), pp. 103379. Date of Electronic Publication: 2023 Sep 01.
DOI: 10.1016/j.rbmo.2023.103379
Abstrakt: Research Question: Does rescue in-vitro maturation (IVM) in the presence or absence of cumulus cells, affect the progress of meiosis I, compared with oocytes that mature in vivo?
Design: This prospective study was conducted in a university-affiliated fertility centre. Ninety-five young oocyte donors (mean age 25.57 ± 4.47) with a normal karyotype and no known fertility problems were included. A total of 390 oocytes (116 mature metaphase II [MII] and 274 immature oocytes) were analysed. The immature oocytes underwent rescue IVM in the presence of cumulus cells (CC; IVM+CC; n = 137) or without them (IVM-CC; n = 137), and IVM rate was calculated. Chromosome copy number analysis using next-generation sequencing (NGS) was performed on all rescue IVM oocytes reaching MII as well as those that were mature at the time of initial denudation (in-vivo-matured oocytes [IVO]).
Results: Maturation rates were similar in IVM+CC and IVM-CC oocytes (62.8 versus 71.5%, P = 0.16). Conclusive cytogenetic results were obtained from 65 MII oocytes from the IVM+CC group, 87 from the IVM-CC group, and 99 from the IVO group. Oocyte euploidy rates for the three groups were similar, at 75.4%, 83.9% and 80.8%, respectively (P = 0.42).
Conclusions: The results suggest that culture of germinal vesicle and metaphase I oocytes in the presence of cumulus cells does not improve rates of IVM. In general, the process of rescue IVM does not appear to alter the frequency of oocytes with a normal chromosome copy number.
(Copyright © 2023 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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