Microbial Primer: Transposon directed insertion site sequencing (TraDIS): A high throughput method for linking genotype to phenotype.

Autor:	Warner IA; Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia., Kok WJ; Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia., Martinelli N; Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia., Yang Z; Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia., Goodall ECA; Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia., Henderson I; Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia.
Jazyk:	angličtina
Zdroj:	Microbiology (Reading, England) [Microbiology (Reading)] 2023 Nov; Vol. 169 (11).
DOI:	10.1099/mic.0.001385
Abstrakt:	Genetic screens are a key tool for linking phenotype and genotype. Transposon mutagenesis was one of the first genetic methodologies to associate genetic loci with phenotypes. The advent of next-generation sequencing transformed the use of this technique allowing rapid interrogation of whole genomes for genes that correlate with phenotype. One method is transposon directed insertion-site sequencing (TraDIS). Here we describe the method, recent developments in technology, and the advantages and disadvantages of this method compared to other genetic screening tools.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu