Whole genome sequencing characteristics of Chlamydia psittaci caprine AMK-16 strain, a promising killed whole cell veterinary vaccine candidate against chlamydia infection.

Autor: Feodorova VA; Laboratory for Fundamental and Applied Research, Department for Microbiology and Biotechnology, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov, Russia., Zaitsev SS; Laboratory for Fundamental and Applied Research, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov, Russia., Lyapina AM; Laboratory for Fundamental and Applied Research, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov, Russia., Kichemazova NV; Laboratory for Fundamental and Applied Research, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov, Russia., Saltykov YV; Laboratory for Fundamental and Applied Research, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov, Russia., Khizhnyakova MA; Laboratory for Fundamental and Applied Research, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov, Russia., Evstifeev VV; Laboratory of Viral and Chlamydial Infections, Federal Center for Toxicological, Radiation and Biological Safety, Kazan, Russia.; Department of Microbiology, Virology and Immunology, Kazan State Academy of Veterinary Medicine by N.E. Bauman, Kazan City, Russia., Larionova OS; Laboratory for Fundamental and Applied Research, Department for Microbiology and Biotechnology, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov, Russia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Oct 30; Vol. 18 (10), pp. e0293612. Date of Electronic Publication: 2023 Oct 30 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0293612
Abstrakt: Chlamydia psittaci is a primary zoonotic pathogen with a broad host range causing severe respiratory and reproductive system infection in animals and humans. To reduce the global burden of C. psittaci-associated diseases on animal welfare and health and to control the pathogen spread in husbandry, effective vaccines based on promising vaccine candidate(s) are required. Recently, the caprine C. psittaci AMK-16 strain (AMK-16) demonstrated a high level of protection (up to 80-100%) in outbred mice and pregnant rabbits immunized with these formaldehyde-inactivated bacteria against experimental chlamydial wild-type infection. This study investigated the molecular characteristics of AMK-16 by whole-genome sequencing followed by molecular typing, phylogenetic analysis and detection of main immunodominant protein(s) eliciting the immune response in mouse model. Similarly to other C. psittaci, AMK-16 harbored an extrachromosomal plasmid. The whole-genome phylogenetic analysis proved that AMK-16 strain belonging to ST28 clustered with only C. psittaci but not with Chlamydia abortus strains. However, AMK-16 possessed the insert which resulted from the recombination event as the additional single chromosome region of a 23,100 bp size with higher homology to C. abortus (98.38-99.94%) rather than to C. psittaci (92.06-92.55%). At least six of 16 CDSs were absent in AMK-16 plasticity zone and 41 CDSs in other loci compared with the reference C. psittaci 6BC strain. Two SNPs identified in the AMK-16 ompA sequence resulted in MOMP polymorphism followed by the formation of a novel genotype/subtype including three other C. psittaci strains else. AMK-16 MOMP provided marked specific cellular and humoral immune response in 100% of mice immunized with the inactivated AMK-16 bacteria. Both DnaK and GrpE encoded by the recombination region genes were less immunoreactive, inducing only a negligible T-cell murine immune response, while homologous antibodies could be detected in 50% and 30% of immunized mice, respectively. Thus, AMK-16 could be a promising vaccine candidate for the development of a killed whole cell vaccine against chlamydiosis in livestock.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Feodorova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje