Characterization of Soybean Events with Enhanced Expression of the Microtubule-Associated Protein 65-1 (MAP65-1).

Autor:	Kim P; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A., Mahboob S; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A., Nguyen HT; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A., Eastman S; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A., Fiala O; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A., Sousek M; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A., Gaussoin RE; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A., Brungardt JL; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A., Jackson-Ziems TA; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A., Roston R; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A., Alfano JR; Center for Plant Science Innovation and Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A. (deceased)., Clemente TE; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A., Guo M; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.
Jazyk:	angličtina
Zdroj:	Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2024 Jan; Vol. 37 (1), pp. 62-71. Date of Electronic Publication: 2024 Jan 29.
DOI:	10.1094/MPMI-09-23-0134-R
Abstrakt:	Microtubule-associated protein 65-1 (MAP65-1) protein plays an essential role in plant cellular dynamics through impacting stabilization of the cytoskeleton by serving as a crosslinker of microtubules. The role of MAP65-1 in plants has been associated with phenotypic outcomes in response to various environmental stresses. The Arabidopsis MAP65-1 ( At MAP65-1) is a known virulence target of plant bacterial pathogens and is thus a component of plant immunity. Soybean events were generated that carry transgenic alleles for both At MAP65-1 and Gm MAP65-1, the soybean At MAP65-1 homolog, under control of cauliflower mosaic virus 35S promoter. Both At MAP65-1 and Gm MAP65-1 transgenic soybeans are more resistant to challenges by the soybean bacterial pathogen Pseudomonas syringae pv. glycinea and the oomycete pathogen Phytophthora sojae , but not the soybean cyst nematode, Heterodera glycines. Soybean plants expressing At MAP65-1 and Gm MAP65-1 also display a tolerance to the herbicide oryzalin, which has a mode of action to destabilize microtubules. In addition, Gm MAP65-1-expressing soybean plants show reduced cytosol ion leakage under freezing conditions, hinting that ectopic expression of Gm MAP65-1 may enhance cold tolerance in soybean. Taken together, overexpression of At MAP65-1 and Gm MAP65-1 confers tolerance of soybean plants to various biotic and abiotic stresses. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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