| Autor: |
Byun SA; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea., Kwon YJ; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea., Lee GY; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea., Choi MJ; Microbiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea., Jeong SH; Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 03722, Republic of Korea., Kim D; Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 03722, Republic of Korea., Choi MH; Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 03722, Republic of Korea., Kee SJ; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea., Kim SH; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea., Shin MG; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea., Won EJ; Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea., Shin JH; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea. |
| Abstrakt: |
We analyzed the virulence traits and azole resistance mechanisms of 104 Candida auris isolates collected from 13 Korean hospitals from 1996 to 2022. Of these 104 isolates, 96 (5 blood and 91 ear isolates) belonged to clade II, and 8 (6 blood and 2 other isolates) belonged to clade I. Fluconazole resistance (minimum inhibitory concentration ≥32 mg/L) was observed in 68.8% of clade II and 25.0% of clade I isolates. All 104 isolates were susceptible to amphotericin B and three echinocandins. In 2022, six clade I isolates indicated the first nosocomial C. auris cluster in Korea. Clade II C. auris isolates exhibited reduced thermotolerance at 42 °C, with diminished in vitro competitive growth and lower virulence in the Galleria mellonella model compared to non-clade II isolates. Of the 66 fluconazole-resistant clade II isolates, several amino acid substitutions were identified: Erg11p in 14 (21.2%), Tac1Ap in 2 (3.0%), Tac1Bp in 62 (93.9%), and Tac1Bp F214S in 33 (50.0%). Although there were a limited number of non-clade II isolates studied, our results suggest that clade II C. auris isolates from Korean hospitals might display lower virulence traits than non-clade II isolates, and their primary fluconazole resistance mechanism is linked to Tac1Bp mutations. |
