Syntaxin-4 and SNAP23 are involved in neutrophil degranulation, but not in the release of mitochondrial DNA during NET formation.

Autor:	Gigon L; Institute of Pharmacology, University of Bern, Bern, Switzerland., Fettrelet T; Institute of Pharmacology, University of Bern, Bern, Switzerland., Miholic M; Institute of Pharmacology, University of Bern, Bern, Switzerland.; Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia., McLeish KR; Department of Medicine, University of Louisville School of Medicine, Louisville, KY, United States., Yousefi S; Institute of Pharmacology, University of Bern, Bern, Switzerland., Stojkov D; Institute of Pharmacology, University of Bern, Bern, Switzerland., Simon HU; Institute of Pharmacology, University of Bern, Bern, Switzerland.; Institute of Biochemistry, Brandenburg Medical School, Neuruppin, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2023 Oct 09; Vol. 14, pp. 1272699. Date of Electronic Publication: 2023 Oct 09 (Print Publication: 2023).
DOI:	10.3389/fimmu.2023.1272699
Abstrakt:	Neutrophils are a specialized subset of white blood cells, which have the ability to store pre-formed mediators in their cytoplasmic granules. Neutrophils are well-known effector cells involved in host protection against pathogens through diverse mechanisms such as phagocytosis, degranulation, extracellular traps, and oxidative burst. In this study, we provide evidence highlighting the significance of the SNARE proteins syntaxin-4 and synaptosomal-associated protein (SNAP) 23 in the release of azurophilic granules, specific granules, and the production of reactive oxygen species in human neutrophils. In contrast, the specific blockade of either syntaxin-4 or SNAP23 did not prevent the release of mitochondrial dsDNA in the process of neutrophil extracellular trap (NET) formation. These findings imply that degranulation and the release of mitochondrial dsDNA involve at least partially distinct molecular pathways in neutrophils. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Gigon, Fettrelet, Miholic, McLeish, Yousefi, Stojkov and Simon.)
Databáze:	MEDLINE
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