Relationship between the Rod complex and peptidoglycan structure in Escherichia coli.

Autor: Ago R; Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan., Tahara YO; Graduate School of Science, Osaka Metropolitan University, Osaka, Japan.; The OMU Advanced Research Center for Natural Science and Technology, Osaka Metropolitan University, Osaka, Japan., Yamaguchi H; Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan., Saito M; Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan., Ito W; Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan., Yamasaki K; Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan., Kasai T; Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan., Okamoto S; Microbial Physiology Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics, Mishima, Shizuoka, Japan., Chikada T; Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan., Oshima T; Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan., Osaka I; Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan., Miyata M; Graduate School of Science, Osaka Metropolitan University, Osaka, Japan.; The OMU Advanced Research Center for Natural Science and Technology, Osaka Metropolitan University, Osaka, Japan., Niki H; Microbial Physiology Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics, Mishima, Shizuoka, Japan.; Department of Genetics, The Graduate University for Advanced Studies, SOKENDAI, Mishima, Shizuoka, Japan., Shiomi D; Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan.
Jazyk: angličtina
Zdroj: MicrobiologyOpen [Microbiologyopen] 2023 Oct; Vol. 12 (5), pp. e1385.
DOI: 10.1002/mbo3.1385
Abstrakt: Peptidoglycan for elongation in Escherichia coli is synthesized by the Rod complex, which includes RodZ. Although various mutant strains of the Rod complex have been isolated, the relationship between the activity of the Rod complex and the overall physical and chemical structures of the peptidoglycan have not been reported. We constructed a RodZ mutant, termed RMR, and analyzed the growth rate, morphology, and other characteristics of cells producing the Rod complexes containing RMR. The growth and morphology of RMR cells were abnormal, and we isolated suppressor mutants from RMR cells. Most of the suppressor mutations were found in components of the Rod complex, suggesting that these suppressor mutations increase the integrity and/or the activity of the Rod complex. We purified peptidoglycan from wild-type, RMR, and suppressor mutant cells and observed their structures in detail. We found that the peptidoglycan purified from RMR cells had many large holes and different compositions of muropeptides from those of WT cells. The Rod complex may be a determinant not only for the whole shape of peptidoglycan but also for its highly dense structure to support the mechanical strength of the cell wall.
(© 2023 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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