Odor-evoked transcriptomics of Aedes aegypti mosquitoes.

Autor: Mappin F; Department of Biological Sciences & Biomolecular Sciences Institute, Florida International University, Miami, Florida, United States of America., Bellantuono AJ; Department of Biological Sciences & Biomolecular Sciences Institute, Florida International University, Miami, Florida, United States of America., Ebrahimi B; Department of Biological Sciences & Biomolecular Sciences Institute, Florida International University, Miami, Florida, United States of America., DeGennaro M; Department of Biological Sciences & Biomolecular Sciences Institute, Florida International University, Miami, Florida, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Oct 24; Vol. 18 (10), pp. e0293018. Date of Electronic Publication: 2023 Oct 24 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0293018
Abstrakt: Modulation of odorant receptors mRNA induced by prolonged odor exposure is highly correlated with ligand-receptor interactions in Drosophila as well as mammals of the Muridae family. If this response feature is conserved in other organisms, this presents an intriguing initial screening tool when searching for novel receptor-ligand interactions in species with predominantly orphan olfactory receptors. We demonstrate that mRNA modulation in response to 1-octen-3-ol odor exposure occurs in a time- and concentration-dependent manner in Aedes aegypti mosquitoes. To investigate gene expression patterns at a global level, we generated an odor-evoked transcriptome associated with 1-octen-3-ol odor exposure. Transcriptomic data revealed that ORs and OBPs were transcriptionally responsive whereas other chemosensory gene families showed little to no differential expression. Alongside chemosensory gene expression changes, transcriptomic analysis found that prolonged exposure to 1-octen-3-ol modulated xenobiotic response genes, primarily members of the cytochrome P450, insect cuticle proteins, and glucuronosyltransferases families. Together, these findings suggest that mRNA transcriptional modulation of olfactory receptors caused by prolonged odor exposure is pervasive across taxa and can be accompanied by the activation of xenobiotic responses.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Mappin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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