Mucin levels in glands of the colonic mucosa of rats with diversion colitis subjected to enemas containing sucralfate and n-acetylcysteine alone or in combination.

Autor:	Brasil VP; Universidade Estadual de Campinas - Postgraduate Program in Surgical Sciences - Campinas (São Paulo) - Brazil., Siqueira RM; Universidade Estadual de Campinas - Postgraduate Program in Surgical Sciences - Campinas (São Paulo) - Brazil., Campos FG; Universidade de São Paulo - Department of Gastroenterology - Faculty of Medicine - São Paulo (São Paulo) - Brazil., Yoshitani MM; Universidade São Francisco - Faculty of Medicine - Medical School - Bragança Paulista (São Paulo) - Brazil., Pereira GP; Universidade São Francisco - Faculty of Medicine - Medical School - Bragança Paulista (São Paulo) - Brazil., Mendonça RLDS; Universidade São Francisco - Faculty of Medicine - Medical School - Bragança Paulista (São Paulo) - Brazil., Kanno DT; Universidade São Francisco - Faculty of Medicine - Medical School - Bragança Paulista (São Paulo) - Brazil., Pereira JA; Universidade São Francisco - Faculty of Medicine - Medical School - Bragança Paulista (São Paulo) - Brazil., Martinez CAR; Universidade Estadual de Campinas - Postgraduate Program in Surgical Sciences - Campinas (São Paulo) - Brazil.; Universidade São Francisco - Faculty of Medicine - Medical School - Bragança Paulista (São Paulo) - Brazil.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2023 Oct 13; Vol. 38, pp. e384023. Date of Electronic Publication: 2023 Oct 13 (Print Publication: 2023).
DOI:	10.1590/acb384023
Abstrakt:	Purpose: To evaluate the tissue content of neutral and acidic mucins, sulfomucins and sialomucins in colonic glands devoid of intestinal transit after enemas containing sucralfate and n-acetylcysteine alone or in combination. Methods: Sixty-four rats underwent intestinal transit bypass. A colonic segment was collected to compose the white group (without intervention). After derivation, the animals were divided into two groups according to whether enemas were performed daily for two or four weeks. Each group was subdivided into four subgroups according to the substance used: control group: saline 0.9%; sucralfate group (SCF): SCF 2 g/kg/day; n-acetylcysteine group (NAC): NAC 100 mg/kg/day; and SCF+NAC group: SCF 2 g/kg/day + NAC 100 mg/kg/day.Neutral and acidic mucins were stained by periodic acid-Schiff and alcian-blue techniques, respectively. The distinction between sulfomucins and sialomucin was made by the high alcian-blue iron diamine technique. The content of mucins in the colonic glands was measured by computerized morphometry. The inflammatory score was assessed using a validated scale. The results between the groups were compared by the Mann-Whitney's test, while the variation according to time by the Kruskal-Wallis' test (Dunn's post-test). A significance level of 5% was adopted. Results: There was reduction in the inflammatory score regardless of the application of isolated or associated substances. Intervention with SCF+NAC increased the content of all mucin subtypes regardless of intervention time. Conclusions: The application of SCF+NAC reduced the inflammatory process of the colonic mucosa and increased the content of different types of mucins in the colonic glands of segments excluded from fecal transit.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu