Fucoidan-loaded electrospun Polyvinyl-alcohol/Chitosan nanofibers with enhanced antibacterial activity for skin tissue engineering.

Autor: Puigmal AC; Bioengineering Institute of Technology (BIT), Universitat Internacional de Catalunya (UIC), Barcelona, 08017, Spain., Ayran M; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Turkey; Institute of Pure and Applied Sciences, Marmara University, Department of Metallurgical and Materials Engineering, Istanbul, Turkey., Ulag S; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Turkey; Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul, Turkey., Altan E; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Turkey; Institute of Pure and Applied Sciences, Marmara University, Department of Metallurgical and Materials Engineering, Istanbul, Turkey., Guncu MM; Department of Medical Microbiology, Faculty of Medicine, Marmara University, Istanbul, Turkey., Aksu B; Department of Medical Microbiology, Faculty of Medicine, Marmara University, Istanbul, Turkey., Durukan BK; Department of Metallurgical and Materials Engineering, Atilim University, Ankara, Turkey., Sasmazel HT; Department of Metallurgical and Materials Engineering, Atilim University, Ankara, Turkey., Perez RA; Bioengineering Institute of Technology (BIT), Universitat Internacional de Catalunya (UIC), Barcelona, 08017, Spain., Koc E; Pharma-Assist Ltd, Unit 16, Carrigeen Business Park, Powerstown, Clonmel Co., Tipperary, Ireland., O'Callaghan D; Pharma-Assist Ltd, Unit 16, Carrigeen Business Park, Powerstown, Clonmel Co., Tipperary, Ireland., Gunduz O; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Turkey; Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul, Turkey. Electronic address: ucemogu@ucl.ac.uk.
Jazyk: angličtina
Zdroj: Journal of the mechanical behavior of biomedical materials [J Mech Behav Biomed Mater] 2023 Dec; Vol. 148, pp. 106163. Date of Electronic Publication: 2023 Oct 07.
DOI: 10.1016/j.jmbbm.2023.106163
Abstrakt: The polymeric nanofiber may interact and control certain regeneration processes at the molecular level to repair damaged tissues. This research focuses on the development of characterization and antibacterial capabilities of polyvinyl alcohol (PVA)/chitosan (CS) nanofibres containing fucoidan (FUC) for tissue engineering as a skin tissue substitute. A control group consisting of 13% PVA/(0.1)% CS nanofiber was prepared. To confer antibacterial properties to the nanofiber, 10, 20, and 30 mg of FUC were incorporated into this control group. The scanning electron microscope (SEM) proved the homogeneous and beadless structures of the nanofibers. The antibacterial activity of the 13% PVA/(0.1)% CS/(10, 20, 30) FUC was tested against the S.aureus and E.coli and the results showed that with FUC addition, the antibacterial activities of the nanofibers increased. The biocompatibility test was performed with a fibroblast cell line for 1, 3, and 7 days of incubation and the results demonstrated that FUC addition enhanced the bioactivity of the 13% PVA/(0.1)% CS nanofibers. In addition, the biocompatibility results showed that 13% PVA/(0.1)% CS/10 FUC had the highest viability value for all incubation periods compared to the others. In addition, the tensile test results showed that; the maximum tensile strength value was observed for 13% PVA/(0.1)% CS/10 FUC nanofibers.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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